Generation of a Synthetic Human Chromosome with Two Centromeric Domains for Advanced Epigenetic Engineering Studies

Elisa Pesenti, Natalay Kouprina, Mikhail Liskovykh, Joan Aurich-Costa, Vladimir Larionov, Hiroshi Masumoto, William C Earnshaw, Oscar Molina

Research output: Contribution to journalArticlepeer-review

Abstract

It is generally accepted that chromatin containing the histone H3 variant CENP-A is an epigenetic mark maintaining centromere identity. However, the pathways leading to the formation and maintenance of centromere chromatin remain poorly characterized due to difficulties of analysis of centromeric repeats in native chromosomes. To address this problem, in our previous studies we generated a human artificial chromosome (HAC) whose centromere contains a synthetic alpha-satellite (alphoid) DNA array containing the tetracycline operator, the alphoidtetO-HAC. The presence of tetO sequences allows the specific targeting of the centromeric region in the HAC with different chromatin modifiers fused to the tetracycline repressor. The alphoidtetO-HAC has been extensively used to investigate protein interactions within the kinetochore and to define the epigenetic signature of centromeric chromatin to maintain a functional kinetochore. In this study, we developed a novel synthetic HAC containing two alphoid DNA arrays with different targeting sequences, tetO, lacO and gal4, the alphoidhybrid-HAC. This new HAC can be used for detailed epigenetic engineering studies because its kinetochore can be simultaneously or independently targeted by different chromatin modifiers and other fusion proteins.

Original languageEnglish
Pages (from-to)1116-1130
Number of pages15
JournalACS Synthetic Biology
Volume7
Issue number4
Early online date22 Mar 2018
DOIs
Publication statusPublished - 20 Apr 2018

Keywords

  • centromere
  • chromosome segregation
  • human artificial chromosome
  • kinetochore
  • mitosis

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