Generation of induced pluripotent stem cells from large domestic animals

Fabiana Fernandes Bressan, Vinícius Bassanezze, Laís Vicari de Figueiredo Pessôa, Chester Bittencourt Sacramento, Tathiane Maistro Malta, Simone Kashima, Paulo Fantinato Neto, Ricardo De Francisco Strefezzi, Naira Caroline Godoy Pieri, José Eduardo Krieger, Dimas Tadeu Covas, Flávio Vieira Meirelles

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

BACKGROUND: Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results.

METHODS: Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation.

RESULTS: Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs).

CONCLUSIONS: The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.

Original languageEnglish
Pages (from-to)1-12
JournalStem Cell Research and Therapy
Volume11
Issue number1
DOIs
Publication statusPublished - 25 Jun 2020

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Animals, Domestic
  • Cattle
  • Cell Differentiation
  • Cellular Reprogramming
  • Embryonic Stem Cells
  • Fibroblasts
  • Horses
  • Induced Pluripotent Stem Cells
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors/genetics
  • Mice
  • Octamer Transcription Factor-3/genetics
  • SOXB1 Transcription Factors/genetics

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