Generation of tissue organoids by compaction reaggregation

Julie M. Sheridan, C. Clare Blackburn

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cellular reaggregation methods are commonly used to generate tissue organoids for use in biological studies. Using a modified method termed "compaction reaggregation," it is possible to establish reaggregates of reproducible size from defined input cell numbers with ease and without specialist equipment. Importantly, this method is suitable for the study of tissues that have proved refractory to reaggregation by other methods. With the option of juxtaposing cell populations, this method is useful for studies of tissue organization and structure.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages143-151
Number of pages9
Volume1092
DOIs
Publication statusPublished - 10 Jan 2014

Publication series

NameMethods in Molecular Biology
Volume1092
ISSN (Print)10643745

Keywords

  • aggregate
  • CoROC
  • organoid
  • reaggregate
  • thymus

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