Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936

Jamie Toombs; Lindsay Panther; Loren Ornelas; Chunyan Liu; Emilda Gomez; Raquel Martin-Ibanez; Simon Cox; Stuart J Ritchie; Sarah Harris; Adele Taylor; Paul Redmond; Tom Russ; Lee Murphy; James D Cooper; Karen Burr; Bhuvaneish Thangaraj Selvaraj; C. Browne; C.N. Svendsen; Sally Cowley; Ian Deary; Siddharthan Chandran; Tara Spires-Jones; Dhruv Sareen

doi:10.1016/j.scr.2020.101851

Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936

Jamie Toombs, Lindsay Panther, Loren Ornelas, Chunyan Liu, Emilda Gomez, Raquel Martin-Ibanez, Simon Cox, Stuart J Ritchie, Sarah Harris, Adele Taylor, Paul Redmond, Tom Russ, Lee Murphy, James D Cooper, Karen Burr, Bhuvaneish Thangaraj Selvaraj, C. Browne, C.N. Svendsen, Sally Cowley, Ian DearySiddharthan Chandran, Tara Spires-Jones, Dhruv Sareen

Research output: Contribution to journal › Article › peer-review

Abstract

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non
integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

Original language	English
Journal	Stem Cell Research
Early online date	20 May 2020
DOIs	https://doi.org/10.1016/j.scr.2020.101851
Publication status	Published - 1 Jul 2020

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10.1016/j.scr.2020.101851

Tackling gaps manuscript (002) updatedAccepted author manuscript, 11.5 MB

The Disconnected Mind Phase 3
Cox, S. & Deary, I.
1/04/16 → 31/03/23
Project: Research
RA2662 Centre for Cognitive Ageing and Cognitive Epidemiology Phase 2.
Porteous, D.
1/09/13 → 31/08/19
Project: Research

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Toombs, J., Panther, L., Ornelas, L., Liu, C., Gomez, E., Martin-Ibanez, R., Cox, S., Ritchie, S. J., Harris, S., Taylor, A., Redmond, P., Russ, T., Murphy, L., Cooper, J. D., Burr, K., Thangaraj Selvaraj, B., Browne, C., Svendsen, C. N., Cowley, S., ... Sareen, D. (2020). Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936. Stem Cell Research. https://doi.org/10.1016/j.scr.2020.101851

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title = "Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936",

abstract = "Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using nonintegrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.",

author = "Jamie Toombs and Lindsay Panther and Loren Ornelas and Chunyan Liu and Emilda Gomez and Raquel Martin-Ibanez and Simon Cox and Ritchie, {Stuart J} and Sarah Harris and Adele Taylor and Paul Redmond and Tom Russ and Lee Murphy and Cooper, {James D} and Karen Burr and {Thangaraj Selvaraj}, Bhuvaneish and C. Browne and C.N. Svendsen and Sally Cowley and Ian Deary and Siddharthan Chandran and Tara Spires-Jones and Dhruv Sareen",

year = "2020",

month = jul,

day = "1",

doi = "10.1016/j.scr.2020.101851",

language = "English",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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Toombs, J, Panther, L, Ornelas, L, Liu, C, Gomez, E, Martin-Ibanez, R, Cox, S, Ritchie, SJ, Harris, S, Taylor, A, Redmond, P, Russ, T, Murphy, L, Cooper, JD, Burr, K, Thangaraj Selvaraj, B, Browne, C, Svendsen, CN, Cowley, S, Deary, I , Chandran, S , Spires-Jones, T & Sareen, D 2020, 'Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936', Stem Cell Research. https://doi.org/10.1016/j.scr.2020.101851

TY - JOUR

T1 - Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936

AU - Toombs, Jamie

AU - Panther, Lindsay

AU - Ornelas, Loren

AU - Liu, Chunyan

AU - Gomez, Emilda

AU - Martin-Ibanez, Raquel

AU - Cox, Simon

AU - Ritchie, Stuart J

AU - Harris, Sarah

AU - Taylor, Adele

AU - Redmond, Paul

AU - Russ, Tom

AU - Murphy, Lee

AU - Cooper, James D

AU - Burr, Karen

AU - Thangaraj Selvaraj, Bhuvaneish

AU - Browne, C.

AU - Svendsen, C.N.

AU - Cowley, Sally

AU - Deary, Ian

AU - Chandran, Siddharthan

AU - Spires-Jones, Tara

AU - Sareen, Dhruv

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using nonintegrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

AB - Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using nonintegrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

U2 - 10.1016/j.scr.2020.101851

DO - 10.1016/j.scr.2020.101851

M3 - Article

SN - 1873-5061

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936

Abstract

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Projects

The Disconnected Mind Phase 3

RA2662 Centre for Cognitive Ageing and Cognitive Epidemiology Phase 2.

Cite this