Genetic analysis of the SPRN gene in ruminants reveals polymorphisms in the alanine-rich segment of shadoo protein

Paula Stewart, Cuicui Shen, Deming Zhao, Wilfred Goldmann

Research output: Contribution to journalArticlepeer-review

Abstract

Prion diseases in ruminants, especially sheep scrapie, cannot be fully explained by PRNP genetics, suggesting the influence of a second modulator gene. The SPRN gene is a good candidate for this role. The SPRN gene encodes the shadoo protein (Sho) which has homology to the PRNP gene encoding prion protein (PrP). Murine Sho has a similar neuroprotective activity to PrP and SPRN gene variants are associated with human prion disease susceptibility. SPRN gene sequences were obtained from 14 species in the orders Artiodactyla and Rodentia. We report here the sequences of more than 20 different Sho proteins that have arisen due to single amino acid substitutions and amino acid deletions or insertions. All Sho sequences contained an alanine-rich sequence homologous to a hydrophobic region with amyloidogenic characteristics in PrP. In contrast with PrP, the Sho sequence showed variability in the number of alanine residues.
Original languageEnglish
Pages (from-to)2575-80
Number of pages6
JournalJournal of General Virology
Volume90
Issue numberPt 10
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Amino Acid Sequence
  • Animals
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Polymorphism, Genetic
  • Ruminants

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