Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

T M Skinner, N L Lopez-Corrales, S I Anderson, A S Loudon, S Lowden, C S Haley, A L Archibald

Research output: Contribution to journalArticlepeer-review

Abstract

The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.
Original languageEnglish
Pages (from-to)82-4
Number of pages3
JournalCytogenetics and Cell Genetics
Volume95
Issue number1-2
DOIs
Publication statusPublished - 2001

Keywords

  • Animals
  • Cell Cycle Proteins
  • Chromosome Mapping
  • Conserved Sequence
  • Exons
  • Gene Expression Profiling
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lod Score
  • Microsatellite Repeats
  • Nuclear Proteins
  • Period Circadian Proteins
  • Physical Chromosome Mapping
  • Polymorphism, Genetic
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine
  • Synteny

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