Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

T M Skinner; N L Lopez-Corrales; S I Anderson; A S Loudon; S Lowden; C S Haley; A L Archibald

doi:10.1159/000057022

Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

T M Skinner, N L Lopez-Corrales, S I Anderson, A S Loudon, S Lowden, C S Haley, A L Archibald

Research output: Contribution to journal › Article › peer-review

Abstract

The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.

Original language	English
Pages (from-to)	82-4
Number of pages	3
Journal	Cytogenetics and Cell Genetics
Volume	95
Issue number	1-2
DOIs	https://doi.org/10.1159/000057022
Publication status	Published - 2001

Keywords

Animals
Cell Cycle Proteins
Chromosome Mapping
Conserved Sequence
Exons
Gene Expression Profiling
Humans
In Situ Hybridization, Fluorescence
Lod Score
Microsatellite Repeats
Nuclear Proteins
Period Circadian Proteins
Physical Chromosome Mapping
Polymorphism, Genetic
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Swine
Synteny

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10.1159/000057022

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@article{5a117b3747814edc89b10357ca180e5c,

title = "Genetic and physical mapping, expression analysis and partial sequence of porcine PER1",

abstract = "The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.",

keywords = "Animals, Cell Cycle Proteins, Chromosome Mapping, Conserved Sequence, Exons, Gene Expression Profiling, Humans, In Situ Hybridization, Fluorescence, Lod Score, Microsatellite Repeats, Nuclear Proteins, Period Circadian Proteins, Physical Chromosome Mapping, Polymorphism, Genetic, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Swine, Synteny",

author = "Skinner, {T M} and Lopez-Corrales, {N L} and Anderson, {S I} and Loudon, {A S} and S Lowden and Haley, {C S} and Archibald, {A L}",

year = "2001",

doi = "10.1159/000057022",

language = "English",

volume = "95",

pages = "82--4",

journal = "Cytogenetics and Cell Genetics",

issn = "0301-0171",

publisher = "S. Karger AG",

number = "1-2",

}

TY - JOUR

T1 - Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

AU - Skinner, T M

AU - Lopez-Corrales, N L

AU - Anderson, S I

AU - Loudon, A S

AU - Lowden, S

AU - Haley, C S

AU - Archibald, A L

PY - 2001

Y1 - 2001

N2 - The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.

AB - The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.

KW - Animals

KW - Cell Cycle Proteins

KW - Chromosome Mapping

KW - Conserved Sequence

KW - Exons

KW - Gene Expression Profiling

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Lod Score

KW - Microsatellite Repeats

KW - Nuclear Proteins

KW - Period Circadian Proteins

KW - Physical Chromosome Mapping

KW - Polymorphism, Genetic

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Swine

KW - Synteny

U2 - 10.1159/000057022

DO - 10.1159/000057022

M3 - Article

C2 - 11978975

VL - 95

SP - 82

EP - 84

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

SN - 0301-0171

IS - 1-2

ER -

Genetic and physical mapping, expression analysis and partial sequence of porcine PER1

Abstract

Keywords

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