Genetic characterization of canine parvovirus from dogs in Pakistan

M. Z. Shabbir; M. U. Sohail; Umer Naveed Chaudhry; W. Yaqub; I. Rashid; M. H. Saleem; M. Munir

doi:10.4149/av_2017_02_06

Genetic characterization of canine parvovirus from dogs in Pakistan

M. Z. Shabbir^*, M. U. Sohail, Umer Naveed Chaudhry, W. Yaqub, I. Rashid, M. H. Saleem, M. Munir

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2-6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64.71%) were found positive both in hemagglutination and PCR assays. Phylogenetic and evolutionary analysis demonstrated higher genetic heterogeneity in studied strains and constituted seven clusters within the CPV-2a group, however, they shared high level of identity with Chinese strains. Further studies are necessary to elucidate genetic analysis and epidemiology of CPV variants across a wide geographical area of the country.

Original language	English
Pages (from-to)	175-182
Number of pages	8
Journal	Acta Virologica
Volume	61
Issue number	2
DOIs	https://doi.org/10.4149/av_2017_02_06
Publication status	Published - 19 May 2017

Keywords / Materials (for Non-textual outputs)

Canine parvovirus
CPV-2a
Dog
Pakistan

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title = "Genetic characterization of canine parvovirus from dogs in Pakistan",

abstract = "Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2-6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64.71%) were found positive both in hemagglutination and PCR assays. Phylogenetic and evolutionary analysis demonstrated higher genetic heterogeneity in studied strains and constituted seven clusters within the CPV-2a group, however, they shared high level of identity with Chinese strains. Further studies are necessary to elucidate genetic analysis and epidemiology of CPV variants across a wide geographical area of the country.",

keywords = "Canine parvovirus, CPV-2a, Dog, Pakistan",

author = "Shabbir, {M. Z.} and Sohail, {M. U.} and Chaudhry, {Umer Naveed} and W. Yaqub and I. Rashid and Saleem, {M. H.} and M. Munir",

year = "2017",

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language = "English",

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T1 - Genetic characterization of canine parvovirus from dogs in Pakistan

AU - Shabbir, M. Z.

AU - Sohail, M. U.

AU - Chaudhry, Umer Naveed

AU - Yaqub, W.

AU - Rashid, I.

AU - Saleem, M. H.

AU - Munir, M.

PY - 2017/5/19

Y1 - 2017/5/19

N2 - Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2-6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64.71%) were found positive both in hemagglutination and PCR assays. Phylogenetic and evolutionary analysis demonstrated higher genetic heterogeneity in studied strains and constituted seven clusters within the CPV-2a group, however, they shared high level of identity with Chinese strains. Further studies are necessary to elucidate genetic analysis and epidemiology of CPV variants across a wide geographical area of the country.

AB - Canine parvoviruses (CPV) exist as antigenic variants with varying frequencies and genetic variabilities across the globe. Given the endemicity and high prevalence in Pakistan, we characterized the CPVs originating from dogs-population to elucidate viral diversity and evolution. Fecal samples from clinically diseased pups (n = 17) of different breeds and age (2-6 months) were processed for hemagglutination assay (HA), and later for partial amplification of VP2 gene sequence and amino acid analysis. A total of 11 samples (64.71%) were found positive both in hemagglutination and PCR assays. Phylogenetic and evolutionary analysis demonstrated higher genetic heterogeneity in studied strains and constituted seven clusters within the CPV-2a group, however, they shared high level of identity with Chinese strains. Further studies are necessary to elucidate genetic analysis and epidemiology of CPV variants across a wide geographical area of the country.

KW - Canine parvovirus

KW - CPV-2a

KW - Dog

KW - Pakistan

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U2 - 10.4149/av_2017_02_06

DO - 10.4149/av_2017_02_06

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SN - 0001-723X

VL - 61

SP - 175

EP - 182

JO - Acta Virologica

JF - Acta Virologica

IS - 2

ER -

Genetic characterization of canine parvovirus from dogs in Pakistan

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Keywords / Materials (for Non-textual outputs)

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