Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers

Yacouba Zare; Isidore Houaga; Michel  Kere; Gnanda B.  Isidore; Boureima  Traore; Millogo  Vinsoun; Maurice  Konkobo; Bandaogo  Ousseni; Moussa Zongo; Bougouma-Yameogo M. C.  Valerie; Romdhane  Rekaya; Nianogo A.  Joseph

doi:10.3390/genes13091523

Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers

Yacouba Zare , Isidore Houaga, Michel Kere, Gnanda B. Isidore, Boureima Traore , Millogo Vinsoun, Maurice Konkobo, Bandaogo Ousseni, Moussa Zongo , Bougouma-Yameogo M. C. Valerie, Romdhane Rekaya , Nianogo A. Joseph

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogenetic
relationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (FIS) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (FST) and among-individual differentiation (FIT), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.

Original language	English
Article number	1523
Pages (from-to)	1-13
Number of pages	13
Journal	Genes
Volume	13
Issue number	9
DOIs	https://doi.org/10.3390/genes13091523
Publication status	Published - 24 Aug 2022

Keywords / Materials (for Non-textual outputs)

local chicken
Konde chicken
genetic diversity
microsatellite markers
Burkina Faso

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10.3390/genes13091523Licence: Creative Commons: Attribution (CC-BY)

genes-13-01523-v2Final published version, 1.31 MBLicence: Creative Commons: Attribution (CC-BY)

CTLGH: A global shared data biological sample resource to support productivity improvement for tropical livestock
Freeman, P., Bronsvoort, M., Clark, E., Connelley, T., Hassan, M., McGrew, M., Morrison, L., Prendergast, J., Robert, C., Smith, J., Sparks, N., Watson, M. & Whitelaw, B.
Non-EU-based charities
1/10/15 → 31/03/22
Project: Research

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@article{2dba0bc15ac844968b744ba665bd0003,

title = "Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers",

abstract = "The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogeneticrelationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (FIS) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (FST) and among-individual differentiation (FIT), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.",

keywords = "local chicken, Konde chicken, genetic diversity, microsatellite markers, Burkina Faso",

author = "Yacouba Zare and Isidore Houaga and Michel Kere and Isidore, {Gnanda B.} and Boureima Traore and Millogo Vinsoun and Maurice Konkobo and Bandaogo Ousseni and Moussa Zongo and Valerie, {Bougouma-Yameogo M. C.} and Romdhane Rekaya and Joseph, {Nianogo A.}",

note = "Funding Information: This research was funded in part by the Bill & Melinda Gates Foundation and with UK aid from the UK Foreign, Commonwealth and Development Office (Grant Agreement OPP1127286) under the auspices of the Centre for Tropical Livestock Genetics and Health (CTLGH), established jointly by the University of Edinburgh, SRUC (Scotland{\textquoteright}s Rural College) and the International Livestock Research Institute. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation nor the UK Government. Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = aug,

day = "24",

doi = "10.3390/genes13091523",

language = "English",

volume = "13",

pages = "1--13",

journal = "Genes",

issn = "2073-4425",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "9",

}

TY - JOUR

T1 - Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers

AU - Zare , Yacouba

AU - Houaga, Isidore

AU - Kere, Michel

AU - Isidore, Gnanda B.

AU - Traore , Boureima

AU - Vinsoun, Millogo

AU - Konkobo, Maurice

AU - Ousseni, Bandaogo

AU - Zongo , Moussa

AU - Valerie, Bougouma-Yameogo M. C.

AU - Rekaya , Romdhane

AU - Joseph, Nianogo A.

N1 - Funding Information: This research was funded in part by the Bill & Melinda Gates Foundation and with UK aid from the UK Foreign, Commonwealth and Development Office (Grant Agreement OPP1127286) under the auspices of the Centre for Tropical Livestock Genetics and Health (CTLGH), established jointly by the University of Edinburgh, SRUC (Scotland’s Rural College) and the International Livestock Research Institute. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation nor the UK Government. Publisher Copyright: © 2022 by the authors.

PY - 2022/8/24

Y1 - 2022/8/24

N2 - The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogeneticrelationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (FIS) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (FST) and among-individual differentiation (FIT), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.

AB - The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogeneticrelationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (FIS) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (FST) and among-individual differentiation (FIT), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.

KW - local chicken

KW - Konde chicken

KW - genetic diversity

KW - microsatellite markers

KW - Burkina Faso

U2 - 10.3390/genes13091523

DO - 10.3390/genes13091523

M3 - Article

SN - 2073-4425

VL - 13

SP - 1

EP - 13

JO - Genes

JF - Genes

IS - 9

M1 - 1523

ER -

Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers

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CTLGH: A global shared data biological sample resource to support productivity improvement for tropical livestock

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