Genetic influences on ovulation of primary oocytes in LT/Sv strain mice

Clare A Everett; Catherine A Auchincloss; Matthew H Kaufman; Catherine M Abbott; John D West

doi:10.1530/rep.00325

Genetic influences on ovulation of primary oocytes in LT/Sv strain mice

Clare A Everett, Catherine A Auchincloss, Matthew H Kaufman, Catherine M Abbott, John D West

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

Original language	English
Pages (from-to)	565-71
Number of pages	7
Journal	Reproduction
Volume	128
Issue number	5
DOIs	https://doi.org/10.1530/rep.00325
Publication status	Published - 2004

Keywords / Materials (for Non-textual outputs)

Animals
Chromosomes, Mammalian
Female
Genes, cdc
Genetic Linkage
Meiosis
Metaphase
Mice
Mice, Inbred Strains
Mice, Mutant Strains
Microsatellite Repeats
Oocytes
Ovulation
Species Specificity
X Chromosome

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10.1530/rep.00325

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@article{8215e6a42b4c441a98e817f69e3813e1,

title = "Genetic influences on ovulation of primary oocytes in LT/Sv strain mice",

abstract = "A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.",

keywords = "Animals, Chromosomes, Mammalian, Female, Genes, cdc, Genetic Linkage, Meiosis, Metaphase, Mice, Mice, Inbred Strains, Mice, Mutant Strains, Microsatellite Repeats, Oocytes, Ovulation, Species Specificity, X Chromosome",

author = "Everett, {Clare A} and Auchincloss, {Catherine A} and Kaufman, {Matthew H} and Abbott, {Catherine M} and West, {John D}",

year = "2004",

doi = "10.1530/rep.00325",

language = "English",

volume = "128",

pages = "565--71",

journal = "Reproduction",

issn = "1470-1626",

publisher = "BioScientifica Ltd.",

number = "5",

}

TY - JOUR

T1 - Genetic influences on ovulation of primary oocytes in LT/Sv strain mice

AU - Everett, Clare A

AU - Auchincloss, Catherine A

AU - Kaufman, Matthew H

AU - Abbott, Catherine M

AU - West, John D

PY - 2004

Y1 - 2004

N2 - A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

AB - A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

KW - Animals

KW - Chromosomes, Mammalian

KW - Female

KW - Genes, cdc

KW - Genetic Linkage

KW - Meiosis

KW - Metaphase

KW - Mice

KW - Mice, Inbred Strains

KW - Mice, Mutant Strains

KW - Microsatellite Repeats

KW - Oocytes

KW - Ovulation

KW - Species Specificity

KW - X Chromosome

U2 - 10.1530/rep.00325

DO - 10.1530/rep.00325

M3 - Article

C2 - 15509702

SN - 1470-1626

VL - 128

SP - 565

EP - 571

JO - Reproduction

JF - Reproduction

IS - 5

ER -

Genetic influences on ovulation of primary oocytes in LT/Sv strain mice

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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