Genetic influences on ovulation of primary oocytes in LT/Sv strain mice

Clare A Everett, Catherine A Auchincloss, Matthew H Kaufman, Catherine M Abbott, John D West

Research output: Contribution to journalArticlepeer-review

Abstract

A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.
Original languageEnglish
Pages (from-to)565-71
Number of pages7
JournalReproduction
Volume128
Issue number5
DOIs
Publication statusPublished - 2004

Keywords

  • Animals
  • Chromosomes, Mammalian
  • Female
  • Genes, cdc
  • Genetic Linkage
  • Meiosis
  • Metaphase
  • Mice
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Microsatellite Repeats
  • Oocytes
  • Ovulation
  • Species Specificity
  • X Chromosome

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