Genetical Genomics of Tonic Immobility in the Chicken

Jesper Fogelholm; Samuel Inkabi; Andrey Höglund; Robin Abbey-Lee; Martin Johnsson; Per Jensen; Rie Henriksen; Dominic Wright

doi:10.3390/genes10050341

Genetical Genomics of Tonic Immobility in the Chicken

Jesper Fogelholm, Samuel Inkabi, Andrey Höglund, Robin Abbey-Lee, Martin Johnsson, Per Jensen, Rie Henriksen, Dominic Wright

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Identifying the molecular mechanisms of animal behaviour is an enduring goal for researchers. Gaining insight into these mechanisms enables us to gain a greater understanding of behaviour and their genetic control. In this paper, we perform Quantitative Trait Loci (QTL) mapping of tonic immobility behaviour in an advanced intercross line between wild and domestic chickens. Genes located within the QTL interval were further investigated using global expression QTL (eQTL) mapping from hypothalamus tissue, as well as causality analysis. This identified five candidate genes, with the genes PRDX4 and ACOT9 emerging as the best supported candidates. In addition, we also investigated the connection between tonic immobility, meat pH and struggling behaviour, as the two candidate genes PRDX4 and ACOT9 have previously been implicated in controlling muscle pH at slaughter. We did not find any phenotypic correlations between tonic immobility, struggling behaviour and muscle pH in a smaller additional cohort, despite these behaviours being repeatable within-test.

Original language	English
Journal	Genes
Volume	10
Issue number	5
DOIs	https://doi.org/10.3390/genes10050341
Publication status	Published - 7 May 2019

Keywords

QTL
eQTL
tonic immobility
behaviour
domestication
muscle pH

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10.3390/genes10050341

Genetical Genomics of Tonic Immobility in the Chicken
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 274 KBLicence: Creative Commons: Attribution (CC-BY)

Cite this

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title = "Genetical Genomics of Tonic Immobility in the Chicken",

abstract = "Identifying the molecular mechanisms of animal behaviour is an enduring goal for researchers. Gaining insight into these mechanisms enables us to gain a greater understanding of behaviour and their genetic control. In this paper, we perform Quantitative Trait Loci (QTL) mapping of tonic immobility behaviour in an advanced intercross line between wild and domestic chickens. Genes located within the QTL interval were further investigated using global expression QTL (eQTL) mapping from hypothalamus tissue, as well as causality analysis. This identified five candidate genes, with the genes PRDX4 and ACOT9 emerging as the best supported candidates. In addition, we also investigated the connection between tonic immobility, meat pH and struggling behaviour, as the two candidate genes PRDX4 and ACOT9 have previously been implicated in controlling muscle pH at slaughter. We did not find any phenotypic correlations between tonic immobility, struggling behaviour and muscle pH in a smaller additional cohort, despite these behaviours being repeatable within-test.",

keywords = "QTL, eQTL, tonic immobility, behaviour, domestication, muscle pH",

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T1 - Genetical Genomics of Tonic Immobility in the Chicken

AU - Fogelholm, Jesper

AU - Inkabi, Samuel

AU - Höglund, Andrey

AU - Abbey-Lee, Robin

AU - Johnsson, Martin

AU - Jensen, Per

AU - Henriksen, Rie

AU - Wright, Dominic

PY - 2019/5/7

Y1 - 2019/5/7

N2 - Identifying the molecular mechanisms of animal behaviour is an enduring goal for researchers. Gaining insight into these mechanisms enables us to gain a greater understanding of behaviour and their genetic control. In this paper, we perform Quantitative Trait Loci (QTL) mapping of tonic immobility behaviour in an advanced intercross line between wild and domestic chickens. Genes located within the QTL interval were further investigated using global expression QTL (eQTL) mapping from hypothalamus tissue, as well as causality analysis. This identified five candidate genes, with the genes PRDX4 and ACOT9 emerging as the best supported candidates. In addition, we also investigated the connection between tonic immobility, meat pH and struggling behaviour, as the two candidate genes PRDX4 and ACOT9 have previously been implicated in controlling muscle pH at slaughter. We did not find any phenotypic correlations between tonic immobility, struggling behaviour and muscle pH in a smaller additional cohort, despite these behaviours being repeatable within-test.

AB - Identifying the molecular mechanisms of animal behaviour is an enduring goal for researchers. Gaining insight into these mechanisms enables us to gain a greater understanding of behaviour and their genetic control. In this paper, we perform Quantitative Trait Loci (QTL) mapping of tonic immobility behaviour in an advanced intercross line between wild and domestic chickens. Genes located within the QTL interval were further investigated using global expression QTL (eQTL) mapping from hypothalamus tissue, as well as causality analysis. This identified five candidate genes, with the genes PRDX4 and ACOT9 emerging as the best supported candidates. In addition, we also investigated the connection between tonic immobility, meat pH and struggling behaviour, as the two candidate genes PRDX4 and ACOT9 have previously been implicated in controlling muscle pH at slaughter. We did not find any phenotypic correlations between tonic immobility, struggling behaviour and muscle pH in a smaller additional cohort, despite these behaviours being repeatable within-test.

KW - QTL

KW - eQTL

KW - tonic immobility

KW - behaviour

KW - domestication

KW - muscle pH

U2 - 10.3390/genes10050341

DO - 10.3390/genes10050341

M3 - Article

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VL - 10

JO - Genes

JF - Genes

SN - 2073-4425

IS - 5

ER -

Genetical Genomics of Tonic Immobility in the Chicken

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Keywords

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