Genome-wide in vivo screen identifies novel host regulators of metastatic colonisation

Louise van der Weyden, Mark J. Arends, Andrew D Campbell, Tobias Bald, Hannah Wardle-Jones, Nicola Griggs, Martin Del Castillo Velasco-Herrera, Thomas Tüting, Owen J. Sansom, Natasha A Karp, Simon Clare, Dianne Gleeson, Edward Ryder, Antonella Galli, Elizabeth Tuck, Emma L Cambridge, Thierry Voet, Iain C Macaulay, Kim Wong, The Sanger Mouse Genetics ProjectSarah Spiegel, Anneliese O Speak, David J Adams

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Metastasis is the leading cause of death for cancer patients. This multi-stage process requires tumour cells to survive in the circulation, extravasate at distant sites, then proliferate; it involves contributions from both the tumour cell and tumour microenvironment (‘host’, which includes stromal cells and the immune system1). Studies suggest the early steps of the metastatic process are relatively efficient, with the post-extravasation regulation of tumour growth (‘colonization’) being critical in determining metastatic outcome2. Here we show the results of screening 810 mutant mouse lines using an in vivo assay to identify microenvironmental regulators of metastatic colonization. We identify 23 genes that, when disrupted in mouse, modify the ability of tumour cells to establish metastatic foci, with 19 of these genes not previously demonstrated to play a role in host control of metastasis. The largest reduction in pulmonary metastasis was observed in sphingosine-1-phosphate (S1P) transporter spinster homologue 2 (Spns2)-deficient mice. We demonstrate a novel outcome of S1P-mediated regulation of lymphocyte trafficking, whereby deletion of Spns2, either globally or in a lymphatic endothelial-specific manner, creates a circulating lymphopenia and a higher percentage of effector T cells and natural killer (NK) cells present in the lung. This allows for potent tumour cell killing, and an overall decreased metastatic burden.
Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalNature
Volume541
Issue number7636
Early online date4 Jan 2017
DOIs
Publication statusPublished - 12 Jan 2017

Fingerprint

Dive into the research topics of 'Genome-wide in vivo screen identifies novel host regulators of metastatic colonisation'. Together they form a unique fingerprint.

Cite this