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Abstract / Description of output
BACKGROUND: The degradation of epigenetic control with age is associated with progressive diseases of ageing, including cancers, immunodeficiency and diabetes. Reduced caloric intake slows the effects of ageing and age-related disease in vertebrates and invertebrates, a process potentially mediated by the impact of caloric restriction on epigenetic factors such as DNA methylation. We used whole genome bisulphite sequencing to study how DNA methylation patterns change with diet in a small invertebrate, the crustacean Daphnia magna. Daphnia show the classic response of longer life under caloric restriction (CR), and they reproduce clonally, which permits the study of epigenetic changes in the absence of genetic variation.
RESULTS: Global cytosine followed by guanine (CpG) methylation was 0.7-0.9%, and there was no difference in overall methylation levels between normal and calorie restricted replicates. However, 333 differentially methylated regions (DMRs) were evident between the normally fed and CR replicates post-filtering. Of these 65% were hypomethylated in the CR group, and 35% were hypermethylated in the CR group.
CONCLUSIONS: Our results demonstrate an effect of CR on the genome-wide methylation profile. This adds to a growing body of research in Daphnia magna that demonstrate an epigenomic response to environmental stimuli. Specifically, gene Ontology (GO) term enrichment of genes associated with hyper and hypo-methylated DMRs showed significant enrichment for methylation and acyl-CoA dehydrogenase activity, which are linked to current understanding of their roles in CR in invertebrate model organisms.
Original language | English |
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Pages (from-to) | 197 |
Number of pages | 11 |
Journal | BMC Genomics |
Volume | 20 |
Issue number | 1 |
DOIs | |
Publication status | Published - 8 Mar 2019 |
Keywords / Materials (for Non-textual outputs)
- Bisulphite sequencing
- Caloric restriction
- DNA methylation
- Daphnia
- Differential methylation
- Epigenetics
- Genomics
- Nutrition
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Dive into the research topics of 'Genome-wide methylation is modified by caloric restriction in Daphnia magna'. Together they form a unique fingerprint.Projects
- 1 Finished
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Cross-generational epigenetic effects of age and diet restriction
Little, T., Bayne, E., Blaxter, M. & Buck, A.
1/03/16 → 31/08/18
Project: Research
Profiles
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Thomas Little
- School of Biological Sciences - Personal Chair in Evolutionary Biology
Person: Academic: Research Active