Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens

Dandan Wang; Lizhi Tan; Yihao Zhi; Lina Bu; Yangyang Wang; Zhang Wang; Yulong Guo; Weihua Tian; Chunlin Xu; Donghua Li; Zhuanjian Li; Ruirui Jiang; Ruili Han; Guoxi Li; Yongqiang Wang; Dong Xia; Yadong Tian; Ian Dunn; Xiaoxiang Hu; Hong Li; Yiqiang Zhao; Xiangtao Kang; Xiaojun Liu

doi:10.1038/s41467-024-50809-9

Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens

Dandan Wang, Lizhi Tan, Yihao Zhi, Lina Bu, Yangyang Wang, Zhang Wang, Yulong Guo, Weihua Tian, Chunlin Xu, Donghua Li, Zhuanjian Li, Ruirui Jiang, Ruili Han, Guoxi Li, Yongqiang Wang, Dong Xia, Yadong Tian, Ian Dunn, Xiaoxiang Hu, Hong LiYiqiang Zhao, Xiangtao Kang, Xiaojun Liu

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Egg-laying performance is of great economic importance in poultry, but the underlying genetic mechanisms are still elusive. In this work, we conduct a multi-omics and multi-tissue integrative study in hens with distinct egg production, to detect the hub candidate genes and construct hub molecular networks contributing to egg-laying phenotypic differences. We identifiy three hub candidate genes as egg-laying facilitators: TFPI2, which promotes the GnRH secretion in hypothalamic neuron cells; CAMK2D, which promotes the FSHβ and LHβ secretion in pituitary cells; and OSTN, which promotes granulosa cell proliferation and the synthesis of sex steroid hormones. We reveal key endocrine factors involving egg production by inter-tissue crosstalk analysis, and demonstrate that both a hepatokine, APOA4, and an adipokine, ANGPTL2, could increase egg production by inter-tissue communication with hypothalamic-pituitary-ovarian axis. Together, These results reveal the molecular mechanisms of multi-tissue coordinative regulation of chicken egg-laying performance and provide key insights to avian reproductive regulation.

Original language	English
Article number	7069
Pages (from-to)	1-18
Number of pages	18
Journal	Nature Communications
Volume	15
Issue number	1
Early online date	16 Aug 2024
DOIs	https://doi.org/10.1038/s41467-024-50809-9
Publication status	Published - Dec 2024

Keywords / Materials (for Non-textual outputs)

Animals
Chickens/genetics
Female
Genome-Wide Association Study
Gonadotropin-Releasing Hormone/metabolism
Granulosa Cells/metabolism
Oviposition/genetics
Pituitary Gland/metabolism
Hypothalamus/metabolism
Reproduction/genetics
Ovary/metabolism
Follicle Stimulating Hormone, beta Subunit/genetics
Angiopoietin-like Proteins/metabolism
Avian Proteins/genetics

Access to Document

10.1038/s41467-024-50809-9

s41467-024-50809-9Final published version, 8.55 MBLicence: Creative Commons: Attribution (CC-BY)

Defining genome activity
Headon, D.
1/04/23 → 31/03/28
Project: Research
Identifying the genomic basis of complex traits in farmed animals
Headon, D.
1/04/23 → 31/03/28
Project: Research

Cite this

Wang, D., Tan, L., Zhi, Y., Bu, L., Wang, Y., Wang, Z., Guo, Y., Tian, W., Xu, C., Li, D., Li, Z., Jiang, R., Han, R., Li, G., Wang, Y., Xia, D., Tian, Y., Dunn, I., Hu, X., ... Liu, X. (2024). Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens. Nature Communications, 15(1), 1-18. Article 7069. https://doi.org/10.1038/s41467-024-50809-9

@article{e74cac1a11354eaab2a4cfde0382a09d,

title = "Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens",

abstract = "Egg-laying performance is of great economic importance in poultry, but the underlying genetic mechanisms are still elusive. In this work, we conduct a multi-omics and multi-tissue integrative study in hens with distinct egg production, to detect the hub candidate genes and construct hub molecular networks contributing to egg-laying phenotypic differences. We identifiy three hub candidate genes as egg-laying facilitators: TFPI2, which promotes the GnRH secretion in hypothalamic neuron cells; CAMK2D, which promotes the FSHβ and LHβ secretion in pituitary cells; and OSTN, which promotes granulosa cell proliferation and the synthesis of sex steroid hormones. We reveal key endocrine factors involving egg production by inter-tissue crosstalk analysis, and demonstrate that both a hepatokine, APOA4, and an adipokine, ANGPTL2, could increase egg production by inter-tissue communication with hypothalamic-pituitary-ovarian axis. Together, These results reveal the molecular mechanisms of multi-tissue coordinative regulation of chicken egg-laying performance and provide key insights to avian reproductive regulation.",

keywords = "Animals, Chickens/genetics, Female, Genome-Wide Association Study, Gonadotropin-Releasing Hormone/metabolism, Granulosa Cells/metabolism, Oviposition/genetics, Pituitary Gland/metabolism, Hypothalamus/metabolism, Reproduction/genetics, Ovary/metabolism, Follicle Stimulating Hormone, beta Subunit/genetics, Angiopoietin-like Proteins/metabolism, Avian Proteins/genetics",

author = "Dandan Wang and Lizhi Tan and Yihao Zhi and Lina Bu and Yangyang Wang and Zhang Wang and Yulong Guo and Weihua Tian and Chunlin Xu and Donghua Li and Zhuanjian Li and Ruirui Jiang and Ruili Han and Guoxi Li and Yongqiang Wang and Dong Xia and Yadong Tian and Ian Dunn and Xiaoxiang Hu and Hong Li and Yiqiang Zhao and Xiangtao Kang and Xiaojun Liu",

note = "{\textcopyright} 2024. The Author(s).",

year = "2024",

month = dec,

doi = "10.1038/s41467-024-50809-9",

language = "English",

volume = "15",

pages = "1--18",

journal = "Nature Communications",

issn = "2041-1723",

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Wang, D, Tan, L, Zhi, Y, Bu, L, Wang, Y, Wang, Z, Guo, Y, Tian, W, Xu, C, Li, D, Li, Z, Jiang, R, Han, R, Li, G, Wang, Y, Xia, D, Tian, Y, Dunn, I, Hu, X, Li, H, Zhao, Y, Kang, X & Liu, X 2024, 'Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens', Nature Communications, vol. 15, no. 1, 7069, pp. 1-18. https://doi.org/10.1038/s41467-024-50809-9

TY - JOUR

T1 - Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens

AU - Wang, Dandan

AU - Tan, Lizhi

AU - Zhi, Yihao

AU - Bu, Lina

AU - Wang, Yangyang

AU - Wang, Zhang

AU - Guo, Yulong

AU - Tian, Weihua

AU - Xu, Chunlin

AU - Li, Donghua

AU - Li, Zhuanjian

AU - Jiang, Ruirui

AU - Han, Ruili

AU - Li, Guoxi

AU - Wang, Yongqiang

AU - Xia, Dong

AU - Tian, Yadong

AU - Dunn, Ian

AU - Hu, Xiaoxiang

AU - Li, Hong

AU - Zhao, Yiqiang

AU - Kang, Xiangtao

AU - Liu, Xiaojun

PY - 2024/12

Y1 - 2024/12

N2 - Egg-laying performance is of great economic importance in poultry, but the underlying genetic mechanisms are still elusive. In this work, we conduct a multi-omics and multi-tissue integrative study in hens with distinct egg production, to detect the hub candidate genes and construct hub molecular networks contributing to egg-laying phenotypic differences. We identifiy three hub candidate genes as egg-laying facilitators: TFPI2, which promotes the GnRH secretion in hypothalamic neuron cells; CAMK2D, which promotes the FSHβ and LHβ secretion in pituitary cells; and OSTN, which promotes granulosa cell proliferation and the synthesis of sex steroid hormones. We reveal key endocrine factors involving egg production by inter-tissue crosstalk analysis, and demonstrate that both a hepatokine, APOA4, and an adipokine, ANGPTL2, could increase egg production by inter-tissue communication with hypothalamic-pituitary-ovarian axis. Together, These results reveal the molecular mechanisms of multi-tissue coordinative regulation of chicken egg-laying performance and provide key insights to avian reproductive regulation.

AB - Egg-laying performance is of great economic importance in poultry, but the underlying genetic mechanisms are still elusive. In this work, we conduct a multi-omics and multi-tissue integrative study in hens with distinct egg production, to detect the hub candidate genes and construct hub molecular networks contributing to egg-laying phenotypic differences. We identifiy three hub candidate genes as egg-laying facilitators: TFPI2, which promotes the GnRH secretion in hypothalamic neuron cells; CAMK2D, which promotes the FSHβ and LHβ secretion in pituitary cells; and OSTN, which promotes granulosa cell proliferation and the synthesis of sex steroid hormones. We reveal key endocrine factors involving egg production by inter-tissue crosstalk analysis, and demonstrate that both a hepatokine, APOA4, and an adipokine, ANGPTL2, could increase egg production by inter-tissue communication with hypothalamic-pituitary-ovarian axis. Together, These results reveal the molecular mechanisms of multi-tissue coordinative regulation of chicken egg-laying performance and provide key insights to avian reproductive regulation.

KW - Animals

KW - Chickens/genetics

KW - Female

KW - Genome-Wide Association Study

KW - Gonadotropin-Releasing Hormone/metabolism

KW - Granulosa Cells/metabolism

KW - Oviposition/genetics

KW - Pituitary Gland/metabolism

KW - Hypothalamus/metabolism

KW - Reproduction/genetics

KW - Ovary/metabolism

KW - Follicle Stimulating Hormone, beta Subunit/genetics

KW - Angiopoietin-like Proteins/metabolism

KW - Avian Proteins/genetics

U2 - 10.1038/s41467-024-50809-9

DO - 10.1038/s41467-024-50809-9

M3 - Article

C2 - 39152103

SN - 2041-1723

VL - 15

SP - 1

EP - 18

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 7069

ER -

Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Defining genome activity

Identifying the genomic basis of complex traits in farmed animals

Cite this