TY - JOUR
T1 - Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance
AU - Global Sewage Surveillance Consortium
AU - Munk, Patrick
AU - Brinch, Christian
AU - Møller, Frederick Duus
AU - Petersen, Thomas N.
AU - Hendriksen, Rene S
AU - Seyfarth, Anne Mette
AU - Kjeldgaard, Jette
AU - Svendsen, Christina Aaby
AU - van Bunnik, Bram A D
AU - Berglund, Fanny
AU - Larsson, D.G. Joakim
AU - Koopmans, Marion P G
AU - Woolhouse, Mark
AU - Aarestrup, Frank M.
N1 - Publisher Copyright:
© 2022. The Author(s).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in 757 sewage samples from 243 cities in 101 countries, collected from 2016 to 2019. We find regional patterns in resistomes, and these differ between subsets corresponding to drug classes and are partly driven by taxonomic variation. The genetic environments of 49 common ARGs are highly diverse, with most common ARGs carried by multiple distinct genomic contexts globally and sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific patterns of dispersal limitation and global transmission. Our data furthermore suggest certain geographies are more prone to transmission events and should receive additional attention.
AB - Antimicrobial resistance (AMR) is a major threat to global health. Understanding the emergence, evolution, and transmission of individual antibiotic resistance genes (ARGs) is essential to develop sustainable strategies combatting this threat. Here, we use metagenomic sequencing to analyse ARGs in 757 sewage samples from 243 cities in 101 countries, collected from 2016 to 2019. We find regional patterns in resistomes, and these differ between subsets corresponding to drug classes and are partly driven by taxonomic variation. The genetic environments of 49 common ARGs are highly diverse, with most common ARGs carried by multiple distinct genomic contexts globally and sometimes on plasmids. Analysis of flanking sequence revealed ARG-specific patterns of dispersal limitation and global transmission. Our data furthermore suggest certain geographies are more prone to transmission events and should receive additional attention.
KW - Anti-Bacterial Agents/pharmacology
KW - Drug Resistance, Bacterial/genetics
KW - Genomics
KW - Metagenome
KW - Sewage
UR - https://doi.org/s41467-023-35890-w
U2 - 10.1038/s41467-022-34312-7
DO - 10.1038/s41467-022-34312-7
M3 - Article
C2 - 36456547
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
M1 - 7251
ER -