TY - JOUR
T1 - Genomic markers associated with antibody response to Newcastle disease virus of Sasso chickens raised in Ethiopia
AU - Girma, M.
AU - Morris, K. M.
AU - Sutton, K.
AU - Esatu, W.
AU - Solomon, B.
AU - Dessie, T.
AU - Psifidi, A.
AU - Vervelde, L.
AU - Hanotte, O.
AU - Banos, G.
AU - Malatji, D. P.
N1 - Funding Information:
The authors would like to acknowledge the following institutions and personnel for funding and facilitating the research. The International Livestock Research Institute (ILRI), Addis Ababa Poultry Research Facility members for their support in chicken monitoring, sample collection, and processing and the staff of the LiveGene program for their technical assistance, supported by the CGIAR Research Program on Livestock (CRP livestock project) sponsored by the CGIAR funding contributors to the Trust Fund (http://www.cgiar.org/about-us/our-funders/). The Bill and Melinda Gates Foundation and the United Kingdom Foreign Commonwealth and Development Office for financial support (Grant Agreement OPP1127286), under the auspices of the Centre for Tropical Livestock Genetics and Health (CTLGH), established jointly by the University of Edinburgh, Scotland’s Rural College (SRUC), and ILRI. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the BMGF nor the UK Government.
Publisher Copyright:
© 2023, South African Journal for Animal Sciences. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Newcastle disease virus (NDV) is one of the highly contagious avian pathogens that threatens poultry producers in endemic zones due to its epidemic potential. Selection for antibody (Ab) response can effectively improve disease resistance in chickens. However, the molecular basis of the variation in Ab response to NDV is unclear. This study aimed to detect genomic markers and genes modulating Ab response to NDV in chickens reared under tropical, outdoor conditions. A genome-wide association study (GWAS) was conducted on Sasso T451A chickens that were naturally exposed to infectious diseases from 56 to 112 days of age to identify regions associated with Ab response to NDV. Phenotypic immune data from 935 chickens, monitored in two batches, and genotyping data of these chickens based low-pass sequencing (2,676,181 single nucleotide polymorphisms, SNPs) were used. BioMart data mining and variant effect predictor tools were used to annotate SNPs and candidate genes, respectively. A total of five SNPs (rs316795557 (FOXP2), chr 1; rs313761644 (CEP170B), chr 5; rs733628728, chr 13; and two unnamed SNPs, chr 30 and chr 33) were associated with the chicken antibody response to NDV at the suggestive significance level. These SNPs are located on chromosomes 1, 5, and 13 and are in genomic regions that contain several genes with roles in the regulation of the immune response. The results of this study pave the path for more investigation into the host immune response of chickens to NDV.
AB - Newcastle disease virus (NDV) is one of the highly contagious avian pathogens that threatens poultry producers in endemic zones due to its epidemic potential. Selection for antibody (Ab) response can effectively improve disease resistance in chickens. However, the molecular basis of the variation in Ab response to NDV is unclear. This study aimed to detect genomic markers and genes modulating Ab response to NDV in chickens reared under tropical, outdoor conditions. A genome-wide association study (GWAS) was conducted on Sasso T451A chickens that were naturally exposed to infectious diseases from 56 to 112 days of age to identify regions associated with Ab response to NDV. Phenotypic immune data from 935 chickens, monitored in two batches, and genotyping data of these chickens based low-pass sequencing (2,676,181 single nucleotide polymorphisms, SNPs) were used. BioMart data mining and variant effect predictor tools were used to annotate SNPs and candidate genes, respectively. A total of five SNPs (rs316795557 (FOXP2), chr 1; rs313761644 (CEP170B), chr 5; rs733628728, chr 13; and two unnamed SNPs, chr 30 and chr 33) were associated with the chicken antibody response to NDV at the suggestive significance level. These SNPs are located on chromosomes 1, 5, and 13 and are in genomic regions that contain several genes with roles in the regulation of the immune response. The results of this study pave the path for more investigation into the host immune response of chickens to NDV.
KW - antibody response
KW - genome-wide association analysis
KW - Newcastle disease
KW - Sasso T451A
KW - vaccine challenge
UR - http://www.scopus.com/inward/record.url?scp=85175371600&partnerID=8YFLogxK
U2 - 10.4314/sajas.v53i5.12
DO - 10.4314/sajas.v53i5.12
M3 - Article
AN - SCOPUS:85175371600
SN - 0375-1589
VL - 53
SP - 728
EP - 736
JO - South african journal of animal science
JF - South african journal of animal science
IS - 5
ER -