Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi

Daniella C. Bartholomeu*, Gustavo C. Cerqueira, Ana Carolina A. Leao, Wanderson D. daRocha, Fabiano S. Pais, Camila Macedo, Appolinaire Djikeng, Santuza M. R. Teixeira, Najib M. El-Sayed

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A novel large multigene family was recently identified in the human pathogen Trypanosoma cruzi, causative agent of Chagas disease, and corresponds to similar to 6% of the parasite diploid genome. The predicted gene products, mucin-associated surface proteins (MASPs), are characterized by highly conserved N- and C-terminal domains and a strikingly variable and repetitive central region. We report here an analysis of the genomic organization and expression profile of masp genes. Masps are not randomly distributed throughout the genome but instead are clustered with genes encoding mucin and other surface protein families. Masp transcripts vary in size, are preferentially expressed during the trypomastigote stage and contain highly conserved 5' and 3' untranslated regions. A sequence analysis of a trypomastigote cDNA library reveals the expression of multiple masp variants with a bias towards a particular masp subgroup. Immunofluorescence assays using antibodies generated against a MASP peptide reveals that the expression of particular MASPs at the cell membrane is limited to subsets of the parasite population. Western blots of phosphatidylinositol-specific phospholipase C (PI-PLC)-treated parasites suggest that MASP may be GPI-anchored and shed into the medium culture, thus contributing to the large repertoire of parasite polypeptides that are exposed to the host immune system.

Original languageEnglish
Pages (from-to)3407-3417
Number of pages11
JournalNucleic Acids Research
Volume37
Issue number10
DOIs
Publication statusPublished - Jun 2009

Keywords

  • CHAGAS-DISEASE
  • MOLECULAR CHARACTERIZATION
  • GENE-EXPRESSION
  • TRANS-SIALIDASE
  • SATELLITE DNA
  • SEQUENCE
  • ANTIGENS
  • PREDICTION
  • ELEMENTS
  • GENERATION

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