Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T cell proliferation

G H Grit, B Devriendt, S Van Coppernolle, T Geurden, J Hope, J Vercruysse, E Cox, P Geldhof, E Claerebout

Research output: Contribution to journalArticlepeer-review

Abstract

Giardia duodenalis is an important intestinal parasite in animals and humans. The role of dendritic cells (DC) in the initiation of the immune response against G. duodenalis is poorly documented. The aim of this study was to test the hypothesis that G. duodenalis interferes with bovine DC function. Therefore the effect of trophozoites and excretion/secretion products on bovine monocyte-derived dendritic cells (MoDC) was investigated. We assessed MoDC maturation and cytokine production of G. duodenalis stimulated MoDC and the ability of these MoDC to take up antigen and induce lymphocyte proliferation. Little or no upregulation of maturation markers CD40 and CD80 was measured, but MHCII expression was increased after stimulation with low parasite concentrations. A dose-dependent decrease of ovalbumin uptake was observed in G. duodenalis stimulated MoDC. In addition, stimulated MoDC induced proliferation of CD3(-) , γδ-T-cells and TCRαβ(+) CD4(+) and -CD8(+) T-cells. Increased transcription of TGF-β was shown in CD4(+) T-cells and increased TNF-α, TGF-β, IL-10, and IL-4 was seen in γδ-T-cells. We found no evidence that G. duodenalis has a regulatory or inhibitory effect on bovine MoDC. MoDC stimulated with G. duodenalis are functionally active and able to induce proliferation of T-cells that produce both pro- and anti-inflammatory cytokines. This article is protected by copyright. All rights reserved.
Original languageEnglish
Pages (from-to)157-169
Number of pages13
JournalParasite Immunology
Volume36
Issue number4
Early online date23 Dec 2013
DOIs
Publication statusPublished - Apr 2014

Keywords

  • cattle
  • dendritic cells
  • Giardia duodenalis
  • maturation
  • T-cell proliferation
  • RESPIRATORY SYNCYTIAL VIRUS
  • IMMUNE-RESPONSE
  • LAMBLIA
  • EXPRESSION
  • CALVES
  • CRYPTOSPORIDIUM
  • LYMPHOCYTES
  • INFECTIONS
  • TOLERANCE
  • MONOCYTE

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