Global gene expression analysis of chicken caecal response to Campylobacter jejuni

Ronan G Shaughnessy; Kieran G Meade; Beatrice A McGivney; Brenda Allan; Cliona O'Farrelly

doi:10.1016/j.vetimm.2011.04.010

Global gene expression analysis of chicken caecal response to Campylobacter jejuni

Ronan G Shaughnessy, Kieran G Meade, Beatrice A McGivney, Brenda Allan, Cliona O'Farrelly

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Abstract / Description of output

Campylobacter jejuni colonises the caecum of more than 90% of commercial chickens. Even though colonisation is asymptomatic, we hypothesised that it is mediated by activation of several biological pathways. We therefore used chicken-specific 20K oligonucleotide microarrays to examine global gene expression in C. jejuni-challenged birds. Microarray results demonstrate small but significant fold-changes in expression of 270 genes 20 h post-challenge, corresponding to a wide range of biological processes including cell growth, nutrient metabolism and immunological activity. Expression of NOX1 (2.3-fold) and VCAM1 (1.5-fold) were significantly increased in colonised birds (P

Original language	English
Pages (from-to)	64-71
Number of pages	8
Journal	Veterinary Immunology and Immunopathology
Volume	142
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetimm.2011.04.010
Publication status	Published - 15 Jul 2011

Keywords / Materials (for Non-textual outputs)

Animals
Campylobacter Infections
Campylobacter jejuni
Cecum
Chickens
Gene Expression Profiling
Gene Expression Regulation
Genes
Immunity
Oligonucleotide Array Sequence Analysis
Poultry Diseases

Access to Document

10.1016/j.vetimm.2011.04.010

Ark Genomics
Watson, M., Archibald, A. & Burt, D.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

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title = "Global gene expression analysis of chicken caecal response to Campylobacter jejuni",

abstract = "Campylobacter jejuni colonises the caecum of more than 90% of commercial chickens. Even though colonisation is asymptomatic, we hypothesised that it is mediated by activation of several biological pathways. We therefore used chicken-specific 20K oligonucleotide microarrays to examine global gene expression in C. jejuni-challenged birds. Microarray results demonstrate small but significant fold-changes in expression of 270 genes 20 h post-challenge, corresponding to a wide range of biological processes including cell growth, nutrient metabolism and immunological activity. Expression of NOX1 (2.3-fold) and VCAM1 (1.5-fold) were significantly increased in colonised birds (P",

keywords = "Animals, Campylobacter Infections, Campylobacter jejuni, Cecum, Chickens, Gene Expression Profiling, Gene Expression Regulation, Genes, Immunity, Oligonucleotide Array Sequence Analysis, Poultry Diseases",

author = "Shaughnessy, {Ronan G} and Meade, {Kieran G} and McGivney, {Beatrice A} and Brenda Allan and Cliona O'Farrelly",

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doi = "10.1016/j.vetimm.2011.04.010",

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TY - JOUR

T1 - Global gene expression analysis of chicken caecal response to Campylobacter jejuni

AU - Shaughnessy, Ronan G

AU - Meade, Kieran G

AU - McGivney, Beatrice A

AU - Allan, Brenda

AU - O'Farrelly, Cliona

PY - 2011/7/15

Y1 - 2011/7/15

N2 - Campylobacter jejuni colonises the caecum of more than 90% of commercial chickens. Even though colonisation is asymptomatic, we hypothesised that it is mediated by activation of several biological pathways. We therefore used chicken-specific 20K oligonucleotide microarrays to examine global gene expression in C. jejuni-challenged birds. Microarray results demonstrate small but significant fold-changes in expression of 270 genes 20 h post-challenge, corresponding to a wide range of biological processes including cell growth, nutrient metabolism and immunological activity. Expression of NOX1 (2.3-fold) and VCAM1 (1.5-fold) were significantly increased in colonised birds (P

AB - Campylobacter jejuni colonises the caecum of more than 90% of commercial chickens. Even though colonisation is asymptomatic, we hypothesised that it is mediated by activation of several biological pathways. We therefore used chicken-specific 20K oligonucleotide microarrays to examine global gene expression in C. jejuni-challenged birds. Microarray results demonstrate small but significant fold-changes in expression of 270 genes 20 h post-challenge, corresponding to a wide range of biological processes including cell growth, nutrient metabolism and immunological activity. Expression of NOX1 (2.3-fold) and VCAM1 (1.5-fold) were significantly increased in colonised birds (P

KW - Animals

KW - Campylobacter Infections

KW - Campylobacter jejuni

KW - Cecum

KW - Chickens

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Genes

KW - Immunity

KW - Oligonucleotide Array Sequence Analysis

KW - Poultry Diseases

U2 - 10.1016/j.vetimm.2011.04.010

DO - 10.1016/j.vetimm.2011.04.010

M3 - Article

C2 - 21605915

SN - 0165-2427

VL - 142

SP - 64

EP - 71

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -

Global gene expression analysis of chicken caecal response to Campylobacter jejuni

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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