Glucose and sucrose signaling modules regulate the arabidopsis juvenile-to-adult phase transition

Lai-Sheng Meng, Qin-Xin Bao, Xin Rong Mu, Chen Tong, Xiao Ying Cao, Jin Jin Huang, Yue Fei, Li Na Xue, Chang Yue Liu, Gary J. Loake

Research output: Contribution to journalArticlepeer-review

Abstract

Cytosolic invertase 1 (CINV1), which converts sucrose into glucose and fructose is a key entry point of carbon into cellar metabolism and Hexokinase 1 (HXK1) functions as a pivotal sensor for glucose. Further, it is well established that sugar signaling can regulate plant gene expression, for example, the Production of Anthocyanin Pigment 1 (PAP1) / MYB75 transcription factor is specifically induced by sucrose accumulation. Exogenous sugars are also thought to trigger the juvenile-to-adult phase transition via a miR156A/SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) module. However, the endogenous factors that regulate this process have remained undetermined. Here we show that sucrose induced expression of the PAP1 transcription factor increases CINV1 activity by direct binding to CINV1 promoter. Further, we identify a glucose feed-forward loop (Sucrose-CINV1-Glucose-HXK1-miR156-SPL9-PAP1-CINV1-Glucose) that controls CINV1 activity to convert sucrose into glucose signaling to dynamically control the juvenile-to-adult phase transition. Moreover, PAP1 directly binds to the SPL9 promoter, activating SPL9 expression, triggering sucrose signaling that mediates the juvenile-to-adult phase transition. Therefore, a glucose signaling feed-forward loop and a sucrose signaling pathway synergistically regulate the Arabidopsis juvenile-to-adult phase transition. Collectively, we identify a molecular link between the major photosynthate sucrose, the entry point of carbon into cellar metabolism and a key developmental process, the juvenile-to-adult phase transition.
Original languageEnglish
Article number109348
Number of pages33
JournalCell Reports
Volume36
Issue number2
Early online date13 Jul 2021
DOIs
Publication statusE-pub ahead of print - 13 Jul 2021

Keywords

  • glucose signaling
  • major photosynthate sucrose
  • juvenile-to-adult transition
  • a glucose feed-forward loop
  • production of anthocyanin pigment 1 (PAP1)
  • cytosolic Invertase 1 (CINV1)
  • squamosa promoter binding protein-like 9 (SPL9)

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