Aim.-The aim of this study was to investigate glycemia balance impact on oxidant-antioxidant status.
Patients and methods.-The study was carried out on 200 patients with type 2 diabetes and 200 healthy subjects. Glycemia, glycated hemoglobin, insulin, hydrogen peroxide, homocysteine, total antioxidant status, superoxide dismutase, glutathione peroxidase and zinc were assayed using colorimetric methods. Thiobarbituric acid reactive substances in LDL were measured by fluoremetric method. LDL polyunsaturated fatty acids quantification was measured by gas chromatography and vitamins A E was by high-performance liquid chromatography. Quantitative insulin check index was calculated by Perseghin et al.'s equation.
Results.-Thiobarbituric acid reactive substances in LDL, hydrogen peroxide and homocysteine significantly increased whereas LDL polyunsaturated fatty acids, total antioxidant status, vitamins A E, zinc, insulin and quantitative insulin check index significantly decreased in diabetes compared to controls. Total antioxidant status and superoxide dismutase activity depended on glycated hemoglobin and hydrogen peroxide. Diabetes group presenting glycated hemoglobin greater than 9% had the smallest insulin concentration and quantitative insulin check index value and the highest oxidant stress parameters.
Conclusion.-Unbalanced glyeaemia produces an oxidant stress which is implicated in insulin sensitivity decrease in type 2 diabetes. (C) 2012 Published by Elsevier Masson SAS.
- Oxidant stress
- Hydrogen peroxide
- Type 2 diabetes