Glycolysis at the climacteric of bananas

K L Ball, J H Green, T ap Rees

Research output: Contribution to journalArticlepeer-review

Abstract

This work was carried out to investigate the relative roles of phosphofructokinase and pyrophosphate-fructose-6-phosphate 1-phosphotransferase during the increased glycolysis at the climacteric in ripening bananas (Musa cavendishii Lamb ex Paxton). Fruit were ripened in the dark in a continuous stream of air in the absence of ethylene. CO2 production, the contents of glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, phosphoenolpyruvate and PPi; and the maximum catalytic activities of pyrophosphate-fructose-6-phosphate 1-phosphotransferase, 6-phosphofructokinase, pyruvate kinase and phosphoenolpyruvate carboxylase were measured over a 12-day period that included the climacteric. Cytosolic fructose-1,6- bisphosphatase could not be detected in extracts of climacteric fruit. The peak of CO2 production was preceded by a threefold rise in phosphofructokinase, and accompanied by falls in fructose 6-phosphate and glucose 6-phosphate, and a rise in fructose 1,6-bisphosphate. No change in pyrophosphate-fructose-6-phosphate 1-phosphotransferase or pyrophosphate was found. It is argued that phosphofructokinase is primarily responsible for the increased entry of fructose 6-phosphate into glycolysis at the climacteric.
Original languageEnglish
Pages (from-to)265-9
Number of pages5
JournalEuropean Journal of Biochemistry
Volume197
Issue number1
Publication statusPublished - 10 Apr 1991

Keywords

  • Carbon Dioxide
  • Fruit
  • Glycolysis
  • Kinetics
  • Phosphoenolpyruvate Carboxylase
  • Phosphofructokinase-1
  • Phosphotransferases
  • Plants
  • Pyruvate Kinase

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