Grafting of genetically modified cells: effects of acetylcholine release in vivo

D Maysinger, P Piccardo, M Goiny, A C Cuello

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In this study, microdialysis was used to investigate functional recovery of central cholinergic neurons in the forebrain of rats with cortical devascularizing lesions. Mature male rats were unilaterally lesioned by disruption of the pia arachnoid vessels and genetically modified fibroblasts secreting nerve growth factor (NGF) were placed at the site of the lesion. One month following surgery, microdialysis probes were installed in the remaining cortex and were perfused with artificial cerebrospinal fluid (csf) containing neostigmine (5 nM) and/or KCl (100 mM). The basal (non-stimulated) release of acetylcholine (ACh) in the cortex was similar in all experimental groups, whereas KCl stimulated release of ACh was significantly augmented (P < 0.05) in the ipsilateral remaining cortex in lesioned animals that have been implanted with fibroblasts secreting NGF. These results suggest that NGF secreted by genetically engineered fibroblasts modulates neuroplasticity in the adult mammalian CNS and may favour recovery of cortical function following injury.

Original languageEnglish
Pages (from-to)543-8
Number of pages6
JournalNeurochemistry International
Volume21
Issue number4
Publication statusPublished - Dec 1992

Keywords / Materials (for Non-textual outputs)

  • Acetylcholine
  • Acetylcholinesterase
  • Animals
  • Cerebral Cortex
  • Dialysis
  • Fibroblasts
  • Genetic Engineering
  • Male
  • Neostigmine
  • Nerve Growth Factors
  • Pia Mater
  • Potassium Chloride
  • Rats
  • Rats, Wistar

Fingerprint

Dive into the research topics of 'Grafting of genetically modified cells: effects of acetylcholine release in vivo'. Together they form a unique fingerprint.

Cite this