Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

Lesley Bell-Sakyi, Edith Paxton, Ulrike G Munderloh, Keith J Sumption

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
Original languageEnglish
Pages (from-to)1238-40
Number of pages3
JournalJournal of Clinical Microbiology
Volume38
Issue number3
Publication statusPublished - Mar 2000

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cattle
  • Cell Line
  • Ehrlichia ruminantium/cytology
  • Ehrlichia ruminantium/growth & development
  • Ehrlichia ruminantium/ultrastructure
  • Heartwater Disease/microbiology
  • Ixodes
  • Microscopy, Electron
  • Vacuoles/microbiology
  • Vacuoles/ultrastructure

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