Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

Lesley Bell-Sakyi; Edith Paxton; Ulrike G Munderloh; Keith J Sumption

Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

Lesley Bell-Sakyi, Edith Paxton, Ulrike G Munderloh, Keith J Sumption

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.

Original language	English
Pages (from-to)	1238-40
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	38
Issue number	3
Publication status	Published - Mar 2000

Keywords

Animals
Cattle
Cell Line
Ehrlichia ruminantium/cytology
Ehrlichia ruminantium/growth & development
Ehrlichia ruminantium/ultrastructure
Heartwater Disease/microbiology
Ixodes
Microscopy, Electron
Vacuoles/microbiology
Vacuoles/ultrastructure

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© 2000, American Society for Microbiology
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title = "Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line",

abstract = "The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.",

keywords = "Animals, Cattle, Cell Line, Ehrlichia ruminantium/cytology, Ehrlichia ruminantium/growth & development, Ehrlichia ruminantium/ultrastructure, Heartwater Disease/microbiology, Ixodes, Microscopy, Electron, Vacuoles/microbiology, Vacuoles/ultrastructure",

author = "Lesley Bell-Sakyi and Edith Paxton and Munderloh, {Ulrike G} and Sumption, {Keith J}",

year = "2000",

month = mar,

language = "English",

volume = "38",

pages = "1238--40",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "3",

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TY - JOUR

T1 - Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

AU - Bell-Sakyi, Lesley

AU - Paxton, Edith

AU - Munderloh, Ulrike G

AU - Sumption, Keith J

PY - 2000/3

Y1 - 2000/3

N2 - The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.

AB - The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.

KW - Animals

KW - Cattle

KW - Cell Line

KW - Ehrlichia ruminantium/cytology

KW - Ehrlichia ruminantium/growth & development

KW - Ehrlichia ruminantium/ultrastructure

KW - Heartwater Disease/microbiology

KW - Ixodes

KW - Microscopy, Electron

KW - Vacuoles/microbiology

KW - Vacuoles/ultrastructure

M3 - Article

VL - 38

SP - 1238

EP - 1240

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -

Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line

Abstract

Keywords

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