Abstract
The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
Original language | English |
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Pages (from-to) | 1238-40 |
Number of pages | 3 |
Journal | Journal of Clinical Microbiology |
Volume | 38 |
Issue number | 3 |
Publication status | Published - Mar 2000 |
Keywords
- Animals
- Cattle
- Cell Line
- Ehrlichia ruminantium/cytology
- Ehrlichia ruminantium/growth & development
- Ehrlichia ruminantium/ultrastructure
- Heartwater Disease/microbiology
- Ixodes
- Microscopy, Electron
- Vacuoles/microbiology
- Vacuoles/ultrastructure