Guidance of primordial germ cell migration by the chemokine SDF-1

Maria Doitsidou; Michal Reichman-Fried; Jürg Stebler; Marion Köprunner; Julia Dörries; Dirk Meyer; Camila V Esguerra; TinChung Leung; Erez Raz

Guidance of primordial germ cell migration by the chemokine SDF-1

Maria Doitsidou, Michal Reichman-Fried, Jürg Stebler, Marion Köprunner, Julia Dörries, Dirk Meyer, Camila V Esguerra, TinChung Leung, Erez Raz

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.

Original language	English
Pages (from-to)	647-59
Number of pages	13
Journal	Cell
Volume	111
Issue number	5
Publication status	Published - 27 Nov 2002

Keywords / Materials (for Non-textual outputs)

Animals
Body Patterning
Cell Movement
Cell Polarity
Chemokine CXCL12
Chemokines, CXC
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Germ Cells
Mutation
Oligonucleotides, Antisense
Protein Biosynthesis
RNA, Messenger
Receptors, CXCR4
Signal Transduction
Tissue Distribution
Zebrafish

Cite this

@article{da966f26cfa64e6799dfc988dff5122d,

title = "Guidance of primordial germ cell migration by the chemokine SDF-1",

abstract = "The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.",

keywords = "Animals, Body Patterning, Cell Movement, Cell Polarity, Chemokine CXCL12, Chemokines, CXC, Embryo, Nonmammalian, Gene Expression Regulation, Developmental, Germ Cells, Mutation, Oligonucleotides, Antisense, Protein Biosynthesis, RNA, Messenger, Receptors, CXCR4, Signal Transduction, Tissue Distribution, Zebrafish",

author = "Maria Doitsidou and Michal Reichman-Fried and J{\"u}rg Stebler and Marion K{\"o}prunner and Julia D{\"o}rries and Dirk Meyer and Esguerra, {Camila V} and TinChung Leung and Erez Raz",

year = "2002",

month = nov,

day = "27",

language = "English",

volume = "111",

pages = "647--59",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - Guidance of primordial germ cell migration by the chemokine SDF-1

AU - Doitsidou, Maria

AU - Reichman-Fried, Michal

AU - Stebler, Jürg

AU - Köprunner, Marion

AU - Dörries, Julia

AU - Meyer, Dirk

AU - Esguerra, Camila V

AU - Leung, TinChung

AU - Raz, Erez

PY - 2002/11/27

Y1 - 2002/11/27

N2 - The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.

AB - The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.

KW - Animals

KW - Body Patterning

KW - Cell Movement

KW - Cell Polarity

KW - Chemokine CXCL12

KW - Chemokines, CXC

KW - Embryo, Nonmammalian

KW - Gene Expression Regulation, Developmental

KW - Germ Cells

KW - Mutation

KW - Oligonucleotides, Antisense

KW - Protein Biosynthesis

KW - RNA, Messenger

KW - Receptors, CXCR4

KW - Signal Transduction

KW - Tissue Distribution

KW - Zebrafish

M3 - Article

C2 - 12464177

SN - 0092-8674

VL - 111

SP - 647

EP - 659

JO - Cell

JF - Cell

IS - 5

ER -

Guidance of primordial germ cell migration by the chemokine SDF-1

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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