H3K36 methylation and DNA-binding both promote Ioc4 recruitment and Isw1b remodeler function

Jian Li, Lena Bergmann, Andreia Rafael de Almeida, Kimberly M Webb, Madelaine M Gogol, Philipp Voigt, Yingfang Liu, Huanhuan Liang, Michaela M Smolle

Research output: Contribution to journalArticlepeer-review

Abstract

The Isw1b chromatin-remodeling complex is specifically recruited to gene bodies to help retain pre-existing histones during transcription by RNA polymerase II. Recruitment is dependent on H3K36 methylation and the Isw1b subunit Ioc4, which contains an N-terminal PWWP domain. Here, we present the crystal structure of the Ioc4-PWWP domain, including a detailed functional characterization of the domain on its own as well as in the context of full-length Ioc4 and the Isw1b remodeler. The Ioc4-PWWP domain preferentially binds H3K36me3-containing nucleosomes. Its ability to bind DNA is required for nucleosome binding. It is also furthered by the unique insertion motif present in Ioc4-PWWP. The ability to bind H3K36me3 and DNA promotes the interaction of full-length Ioc4 with nucleosomes in vitro and they are necessary for its recruitment to gene bodies in vivo. Furthermore, a fully functional Ioc4-PWWP domain promotes efficient remodeling by Isw1b and the maintenance of ordered chromatin in vivo, thereby preventing the production of non-coding RNAs.
Original languageEnglish
Article numbergkac077
Pages (from-to)2549-2565
Number of pages17
JournalNucleic Acids Research
Volume50
Issue number5
Early online date21 Feb 2022
DOIs
Publication statusPublished - 21 Mar 2022

Keywords

  • Chromatin
  • Chromatin Assembly and Disassembly
  • DNA/chemistry
  • Histone Code
  • Methylation
  • Nucleosomes/genetics
  • Protein Binding

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