Heat in optical tweezers

B. del Rosal; P. Haro-Gonzalez; W. T. Ramsay; L. M. Maestro; K. Santacruz-Gomez; M. C. Iglesias-de la Cruz; F. Sanz-Rodriguez; J. Y. Chooi; P. Rodriguez-Sevilla; D. Choudhury; A. K. Kar; J. G. Garcia-Sole; L. Patterson; D. Jaque

doi:10.1117/12.2027750

Heat in optical tweezers

B. del Rosal^*, P. Haro-Gonzalez, W. T. Ramsay, L. M. Maestro, K. Santacruz-Gomez, M. C. Iglesias-de la Cruz, F. Sanz-Rodriguez, J. Y. Chooi, P. Rodriguez-Sevilla, D. Choudhury, A. K. Kar, J. G. Garcia-Sole, L. Patterson, D. Jaque

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract / Description of output

Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 degrees C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

Original language	English
Title of host publication	OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X
Editors	K Dholakia, GC Spalding
Place of Publication	BELLINGHAM
Publisher	SPIE
Number of pages	8
DOIs	https://doi.org/10.1117/12.2027750
Publication status	Published - 2013
Event	Conference on Optical Trapping and Optical Micromanipulation X - San Diego, Canada Duration: 25 Aug 2013 → 29 Aug 2013

Publication series

Name	Proceedings of SPIE
Publisher	SPIE-INT SOC OPTICAL ENGINEERING
Volume	8810
ISSN (Print)	0277-786X

Conference

Conference	Conference on Optical Trapping and Optical Micromanipulation X
Country/Territory	Canada
Period	25/08/13 → 29/08/13

Keywords / Materials (for Non-textual outputs)

Optical trapping
nanothermometry
quantum dots
fluorescence imaging
microspheres
lymphocytes
CELL
MANIPULATION
TRAPS
TEMPERATURE
APOPTOSIS
NECROSIS

Access to Document

10.1117/12.2027750

Cite this

del Rosal, B., Haro-Gonzalez, P., Ramsay, W. T., Maestro, L. M., Santacruz-Gomez, K., Iglesias-de la Cruz, M. C., Sanz-Rodriguez, F., Chooi, J. Y., Rodriguez-Sevilla, P., Choudhury, D., Kar, A. K., Garcia-Sole, J. G., Patterson, L., & Jaque, D. (2013). Heat in optical tweezers. In K. Dholakia, & GC. Spalding (Eds.), OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X (Proceedings of SPIE; Vol. 8810). SPIE. https://doi.org/10.1117/12.2027750

@inproceedings{5f2e6035fb1645fbbff73356b927974a,

title = "Heat in optical tweezers",

abstract = "Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 degrees C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.",

keywords = "Optical trapping, nanothermometry, quantum dots, fluorescence imaging, microspheres, lymphocytes, CELL, MANIPULATION, TRAPS, TEMPERATURE, APOPTOSIS, NECROSIS",

author = "{del Rosal}, B. and P. Haro-Gonzalez and Ramsay, {W. T.} and Maestro, {L. M.} and K. Santacruz-Gomez and {Iglesias-de la Cruz}, {M. C.} and F. Sanz-Rodriguez and Chooi, {J. Y.} and P. Rodriguez-Sevilla and D. Choudhury and Kar, {A. K.} and Garcia-Sole, {J. G.} and L. Patterson and D. Jaque",

year = "2013",

doi = "10.1117/12.2027750",

language = "English",

series = "Proceedings of SPIE",

publisher = "SPIE",

editor = "K Dholakia and GC Spalding",

booktitle = "OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X",

address = "United States",

note = "Conference on Optical Trapping and Optical Micromanipulation X ; Conference date: 25-08-2013 Through 29-08-2013",

}

del Rosal, B, Haro-Gonzalez, P, Ramsay, WT, Maestro, LM, Santacruz-Gomez, K, Iglesias-de la Cruz, MC, Sanz-Rodriguez, F, Chooi, JY, Rodriguez-Sevilla, P, Choudhury, D, Kar, AK, Garcia-Sole, JG, Patterson, L & Jaque, D 2013, Heat in optical tweezers. in K Dholakia & GC Spalding (eds), OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X. Proceedings of SPIE, vol. 8810, SPIE, BELLINGHAM, Conference on Optical Trapping and Optical Micromanipulation X, Canada, 25/08/13. https://doi.org/10.1117/12.2027750

TY - GEN

T1 - Heat in optical tweezers

AU - del Rosal, B.

AU - Haro-Gonzalez, P.

AU - Ramsay, W. T.

AU - Maestro, L. M.

AU - Santacruz-Gomez, K.

AU - Iglesias-de la Cruz, M. C.

AU - Sanz-Rodriguez, F.

AU - Chooi, J. Y.

AU - Rodriguez-Sevilla, P.

AU - Choudhury, D.

AU - Kar, A. K.

AU - Garcia-Sole, J. G.

AU - Patterson, L.

AU - Jaque, D.

PY - 2013

Y1 - 2013

N2 - Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 degrees C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

AB - Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 degrees C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

KW - Optical trapping

KW - nanothermometry

KW - quantum dots

KW - fluorescence imaging

KW - microspheres

KW - lymphocytes

KW - CELL

KW - MANIPULATION

KW - TRAPS

KW - TEMPERATURE

KW - APOPTOSIS

KW - NECROSIS

U2 - 10.1117/12.2027750

DO - 10.1117/12.2027750

M3 - Conference contribution

T3 - Proceedings of SPIE

BT - OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X

A2 - Dholakia, K

A2 - Spalding, GC

PB - SPIE

CY - BELLINGHAM

T2 - Conference on Optical Trapping and Optical Micromanipulation X

Y2 - 25 August 2013 through 29 August 2013

ER -

Heat in optical tweezers

Abstract / Description of output

Publication series

Conference

Keywords / Materials (for Non-textual outputs)

Access to Document

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Cite this