Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts

Cristina F Hickman; Michael Clinton; Alison Ainslie; Cheryl J Ashworth; John A Rooke

doi:10.1111/aji.12131

Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts

Cristina F Hickman, Michael Clinton, Alison Ainslie, Cheryl J Ashworth, John A Rooke

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

PROBLEM: The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. METHOD OF STUDY: In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. RESULTS: Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. CONCLUSION: The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.

Original language	English
Pages (from-to)	177-181
Number of pages	5
Journal	American Journal of Reproductive Immunology
Volume	70
Issue number	3
Early online date	3 May 2013
DOIs	https://doi.org/10.1111/aji.12131
Publication status	Published - Sept 2013

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10.1111/aji.12131

http://onlinelibrary.wiley.com/doi/10.1111/aji.12131/abstract

Control of development and reproductive traits
Burdon, T., Argyle, D., Ashworth, C., Beard, P., Brunton, P., Burt, D., Clinton, M., Dunn, I., Farquharson, C., Headon, D., Hocking, P., Hohenstein, P., Hume, D., Jackson, I., McColl, B., McGrew, M., McLachlan, G., Sang, H., Summers, K. & Whitelaw, B.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

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title = "Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts",

abstract = "PROBLEM: The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. METHOD OF STUDY: In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. RESULTS: Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. CONCLUSION: The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.",

author = "Hickman, {Cristina F} and Michael Clinton and Alison Ainslie and Ashworth, {Cheryl J} and Rooke, {John A}",

note = "{\textcopyright} 2013 John Wiley & Sons Ltd.",

year = "2013",

month = sep,

doi = "10.1111/aji.12131",

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T1 - Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts

AU - Hickman, Cristina F

AU - Clinton, Michael

AU - Ainslie, Alison

AU - Ashworth, Cheryl J

AU - Rooke, John A

PY - 2013/9

Y1 - 2013/9

N2 - PROBLEM: The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. METHOD OF STUDY: In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. RESULTS: Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. CONCLUSION: The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.

AB - PROBLEM: The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. METHOD OF STUDY: In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. RESULTS: Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. CONCLUSION: The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.

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DO - 10.1111/aji.12131

M3 - Article

C2 - 23638875

SN - 1600-0897

VL - 70

SP - 177

EP - 181

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

IS - 3

ER -

Heat Shock Induces Interferon-TAU Gene Expression by In Vitro-Produced Bovine Blastocysts

Abstract

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Control of development and reproductive traits

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