Hemogenic endothelial fate mapping reveals dual developmental origin of mast cells

Rebecca Gentek, Clément Ghigo, Guillaume Hoeffel, Maxime Jacques Bulle, Rasha Msallam, Gregory Gautier, Pierre Launay, Jinmiao Chen, Florent Ginhoux, Marc Bajénoff

Research output: Contribution to journalArticlepeer-review

Abstract

Hematopoiesis occurs in distinct waves. “Definitive” hematopoietic stem cells (HSCs) with the potential for all blood lineages emerge in the aorta-gonado-mesonephros, while “primitive” progenitors, whose potential is thought to be limited to erythrocytes, megakaryocytes, and macrophages, arise earlier in the yolk sac (YS). Here, we questioned whether other YS lineages exist that have not been identified, partially owing to limitations of current lineage tracing models. We established the use of Cdh5-CreERT2 for hematopoietic fate mapping, which revealed the YS origin of mast cells (MCs). YS-derived MCs were replaced by definitive MCs, which maintained themselves independently from the bone marrow in the adult. Replacement occurred with tissue-specific kinetics. MCs in the embryonic skin, but not other organs, remained largely YS derived prenatally and were phenotypically and transcriptomically distinct from definite adult MCs. We conclude that within myeloid lineages, dual hematopoietic origin is shared between macrophages and MCs.

Original languageEnglish
Pages (from-to)1160-1171.e5
JournalImmunity
Volume48
Issue number6
Early online date29 May 2018
DOIs
Publication statusPublished - 19 Jun 2018

Keywords

  • Animals
  • Cell Lineage/immunology
  • Hemangioblasts/cytology
  • Hematopoiesis/physiology
  • Hematopoietic Stem Cells/cytology
  • Macrophages/cytology
  • Mast Cells/cytology
  • Mice
  • Skin/cytology
  • Yolk Sac/cytology

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