Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

Jose Meseguer Ripolles, Yu Wang, Agnes Sorteberg, Aishwariya Sharma, Nan-Linda Ding, Balta Lucendo Villarin, Philipp Krämer, Charis-Patricia Segeritz, David Hay

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.
Original languageEnglish
Article numbere61256
Number of pages8
JournalJournal of Visualized Experiments (JoVE)
Issue number159
DOIs
Publication statusPublished - 10 May 2020

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