Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

Jose Meseguer Ripolles; Yu Wang; Agnes Sorteberg; Aishwariya Sharma; Nan-Linda Ding; Balta Lucendo Villarin; Philipp  Krämer; Charis-Patricia Segeritz; David Hay

doi:10.3791/6125

Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

Jose Meseguer Ripolles, Yu Wang, Agnes Sorteberg, Aishwariya Sharma, Nan-Linda Ding, Balta Lucendo Villarin, Philipp Krämer, Charis-Patricia Segeritz, David Hay

Research output: Contribution to journal › Article › peer-review

Abstract

Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.

Original language	English
Article number	e61256
Number of pages	8
Journal	Journal of Visualized Experiments (JoVE)
Issue number	159
DOIs	https://doi.org/10.3791/6125
Publication status	Published - 10 May 2020

Access to Document

10.3791/6125Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Meseguer-Ripolles et al 2020 JoVEAccepted author manuscript, 68.4 KB
jove-61256-pdfFinal published version, 2.56 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Cite this

@article{b6706315b9e54805bd50a926d04bbf17,

title = "Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System",

abstract = "Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.",

author = "\{Meseguer Ripolles\}, Jose and Yu Wang and Agnes Sorteberg and Aishwariya Sharma and Nan-Linda Ding and \{Lucendo Villarin\}, Balta and Philipp Kr{\"a}mer and Charis-Patricia Segeritz and David Hay",

year = "2020",

month = may,

day = "10",

doi = "10.3791/6125",

language = "English",

journal = "Journal of Visualized Experiments (JoVE)",

issn = "1940-087X",

publisher = "MYJoVE Corporation",

number = "159",

}

TY - JOUR

T1 - Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

AU - Meseguer Ripolles, Jose

AU - Wang, Yu

AU - Sorteberg, Agnes

AU - Sharma, Aishwariya

AU - Ding, Nan-Linda

AU - Lucendo Villarin, Balta

AU - Krämer, Philipp

AU - Segeritz, Charis-Patricia

AU - Hay, David

PY - 2020/5/10

Y1 - 2020/5/10

N2 - Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.

AB - Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.

U2 - 10.3791/6125

DO - 10.3791/6125

M3 - Article

SN - 1940-087X

JO - Journal of Visualized Experiments (JoVE)

JF - Journal of Visualized Experiments (JoVE)

IS - 159

M1 - e61256

ER -

Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

Abstract

Access to Document

Fingerprint

Cite this