Hepatoprotective effects of Biochanin A on thioacetamide-induced liver cirrhosis in experimental rats.

Mohamed Yousif Ibrahim, Zaenah Zuhair Alamri, Ameena S.M. Juma, Sarah Ashour Hamood, Suhayla Hamad Shareef, Mahmood Ameen Abdulla, Soher Jayash

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The protective effect of biochanin A (BCA) on the histopathology, immunohistochemistry, and biochemistry of thioacetamide (TAA)-induced liver cirrhosis in vivo was investigated. There was a significant reduction in liver weight and hepatocyte propagation, with much lower cell injury in rat groups treated with BCA (25 mg/kg and 50 mg/kg) following TAA induction. These groups had significantly lower levels of proliferating cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA). The liver homogenate showed increased antioxidant enzyme activity of super-oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as a decrease in malondialdehyde (MDA) levels. The serum biomarkers associated with liver function, namely alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transaminase (GGT), exhibited a return to normal levels comparable to those observed in both the normal control group and the reference control group. Taken together, the normal microanatomy of hepatocytes, the inhibition of PCNA and α-SMA, improved anti-oxidant enzymes (SOD, CAT and GPx), and condensed MDA with repairs of liver biomarkers validate BCA's hepatoprotective effect.
Original languageEnglish
Article number7608
Pages (from-to)1-14
Number of pages14
JournalMolecules
Volume22
Issue number28
Early online date15 Nov 2023
DOIs
Publication statusE-pub ahead of print - 15 Nov 2023

Keywords / Materials (for Non-textual outputs)

  • Liver cirrhosis
  • TAA
  • Biochanin A
  • Antioxidant enzymes
  • Histopathology
  • Immunohistochemistry

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