High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi

Peter Hohenstein; Joan Slight; Derya Deniz Ozdemir; Sally F Burn; Rachel Berry; Nicholas D Hastie

doi:10.1186/1755-8417-1-3

High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi

Peter Hohenstein, Joan Slight, Derya Deniz Ozdemir, Sally F Burn, Rachel Berry, Nicholas D Hastie

Research output: Contribution to journal › Article › peer-review

Abstract

Rosa26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice. However, the vectors generally used to generate Rosa26 knock-in constructs show instability problems, which have a severe impact on the efficiency of the system.

Original language	English
Pages (from-to)	3
Journal	PathoGenetics
Volume	1
Issue number	1
DOIs	https://doi.org/10.1186/1755-8417-1-3
Publication status	Published - 2008

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10.1186/1755-8417-1-3

High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi
Copyright © 2008 Hohenstein et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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http://www.pathogeneticsjournal.com/content/1/1/3

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title = "High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi",

abstract = "Rosa26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice. However, the vectors generally used to generate Rosa26 knock-in constructs show instability problems, which have a severe impact on the efficiency of the system.",

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AU - Berry, Rachel

AU - Hastie, Nicholas D

PY - 2008

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DO - 10.1186/1755-8417-1-3

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High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi

Abstract

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