High-fat diet disrupts metabolism in two generations of rats in a parent-of-origin specific manner

T. J. G. Chambers, M D Morgan, AH Heger, R. M. Sharpe, A. J. Drake

Research output: Contribution to journalArticlepeer-review

Abstract

Experimental and epidemiological evidence demonstrate that ancestral diet might contribute towards offspring health. This suggests that nutrition may be able to modify genetic or epigenetic information carried by germ cells (GCs). To examine if a parental high fat diet (HFD) influences metabolic health in two generations of offspring, GC-eGFP Sprague Dawley rats were weaned onto HFD (45% fat) or Control Diet (CD; 10% fat). At 19 weeks, founders (F0) were bred with controls, establishing the F1 generation. HFD resulted in 9.7% and 14.7% increased weight gain in male and female F0 respectively. F1 offspring of HFD mothers and F1 daughters of HFD-fed fathers had increased weight gain compared to controls. F1 rats were bred with controls at 19 weeks to generate F2 offspring. F2 male offspring derived from HFD-fed maternal grandfathers exhibited increased adiposity, plasma leptin and luteinising hormone to testosterone ratio. Despite transmission via the founding male germline, we did not find significant changes in the F0 intra-testicular GC transcriptome. Thus, HFD consumption by maternal grandfathers results in a disrupted metabolic and reproductive hormone phenotype in grandsons in the absence of detectable changes in the intra-testicular GC transcriptome.

Original languageEnglish
Article number31857
Number of pages11
JournalScientific Reports
Volume6
Early online date23 Aug 2016
DOIs
Publication statusPublished - 23 Aug 2016

Keywords

  • BODY-MASS INDEX
  • PATERNAL OBESITY
  • OLDER MEN
  • MICE
  • HEALTH
  • HYPOGONADISM
  • INHERITANCE
  • EXPRESSION
  • CONTRIBUTE
  • MAMMALS

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