High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy

Belén Rubio Ruiz; Ana M. Pérez-López; Thomas L. Bray; Martin Lee; Alan Serrels; Martin Prieto; Manuel Arruebo; Neil O. Carragher; Víctor Sebastián; Asier Unciti-Broceta

doi:10.1021/acsami.7b17282

High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy

Belén Rubio Ruiz, Ana M. Pérez-López, Thomas L. Bray, Martin Lee, Alan Serrels, Martin Prieto, Manuel Arruebo, Neil O. Carragher, Víctor Sebastián, Asier Unciti-Broceta

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Herein we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labelled cancer cells upon short exposure to near infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate micro-ablation of cancerous tissue in tumour xenografts in mice.

Original language	English
Journal	ACS Applied Materials & Interfaces
Early online date	10 Jan 2018
DOIs	https://doi.org/10.1021/acsami.7b17282
Publication status	E-pub ahead of print - 10 Jan 2018

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Journal Article

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10.1021/acsami.7b17282

acsami.7b17282Final published version, 4.99 MB

Locally-Controlled Activation of Chemotherapeutics by Palladium Catalysis as a Novel Anticancer Technology
Unciti-Broceta, A. & Brennan, P.
EPSRC
1/04/16 → 30/09/21
Project: Research
R43494 Advancing our capabilities for visualization of cellular structures and isolated complexes byelectron microscopy
Böttcher, B. & Schirmer, E.
UK-based charities
22/06/15 → 21/06/20
Project: Research
Invasion and metastasis; understanding and targeting an adhesion protein network - Programme Grant Renewal
Frame, M.
UK-based charities
1/05/13 → 31/08/18
Project: Research

Asier Unciti-Broceta
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of Medicinal Chemistry
Person: Academic: Research Active

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Rubio Ruiz, B., Pérez-López, A. M., Bray, T. L., Lee, M., Serrels, A., Prieto, M., Arruebo, M., Carragher, N. O., Sebastián, V., & Unciti-Broceta, A. (2018). High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy. ACS Applied Materials & Interfaces. Advance online publication. https://doi.org/10.1021/acsami.7b17282

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title = "High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy",

abstract = "Herein we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labelled cancer cells upon short exposure to near infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate micro-ablation of cancerous tissue in tumour xenografts in mice.",

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author = "{Rubio Ruiz}, Bel{\'e}n and P{\'e}rez-L{\'o}pez, {Ana M.} and Bray, {Thomas L.} and Martin Lee and Alan Serrels and Martin Prieto and Manuel Arruebo and Carragher, {Neil O.} and V{\'i}ctor Sebasti{\'a}n and Asier Unciti-Broceta",

year = "2018",

month = jan,

day = "10",

doi = "10.1021/acsami.7b17282",

language = "English",

journal = "ACS Applied Materials & Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

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T1 - High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy

AU - Rubio Ruiz, Belén

AU - Pérez-López, Ana M.

AU - Bray, Thomas L.

AU - Lee, Martin

AU - Serrels, Alan

AU - Prieto, Martin

AU - Arruebo, Manuel

AU - Carragher, Neil O.

AU - Sebastián, Víctor

AU - Unciti-Broceta, Asier

PY - 2018/1/10

Y1 - 2018/1/10

N2 - Herein we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labelled cancer cells upon short exposure to near infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate micro-ablation of cancerous tissue in tumour xenografts in mice.

AB - Herein we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labelled cancer cells upon short exposure to near infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate micro-ablation of cancerous tissue in tumour xenografts in mice.

KW - Journal Article

U2 - 10.1021/acsami.7b17282

DO - 10.1021/acsami.7b17282

M3 - Article

C2 - 29320154

SN - 1944-8244

JO - ACS Applied Materials & Interfaces

JF - ACS Applied Materials & Interfaces

ER -

High-Precision Photothermal Ablation using Biocompatible Palladium Nanoparticles and Laser Scanning Microscopy

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Projects

Locally-Controlled Activation of Chemotherapeutics by Palladium Catalysis as a Novel Anticancer Technology

R43494 Advancing our capabilities for visualization of cellular structures and isolated complexes byelectron microscopy

Invasion and metastasis; understanding and targeting an adhesion protein network - Programme Grant Renewal

Profiles

Asier Unciti-Broceta

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