High prevalence of high-risk oncogenic human papillomaviruses harboring atypical distribution in women of childbearing age living in Libreville, Gabon

Ali Si-Mohamed, Angélique Ndjoyi-Mbiguino, Kate Cuschieri, Isabelle Ndombi Onas, Isabelle Colombet, Francis Ozouaki, Jérôme Le Goff, Heather Cubie, Laurent Bélec

Research output: Contribution to journalArticlepeer-review

Abstract

The extent of human papillomavirus (HPV) genital shedding and type-specific diversity were evaluated in 354 consecutive women of childbearing age living in Libreville, Gabon. Detection of HPV DNA was performed by PCR using the MY09/MY11 primer set on DNA extracted from endocervical swabs. All PCR positive specimens were subjected to direct sequencing and HPV genotypes were identified on the basis of >95% sequence homology in the L1 region. Reverse line blot hybridization assay was used when a genotype could not be resolved by sequencing alone. HPV DNA was detected in 163 (46%) women, all clinically asymptomatic for HPV-related lesions. The highest prevalence of genital HPV detection (45%) was in the age group from 22 to 29 years. A total of 90 women (55%) harbored high-risk (HR) genotypes, with the most common being HPV-53 (19; 12%), HPV-58 (17; 11%), and HPV-16 (16; 10%). Low-risk genotypes were found in 36 (22%) women with HPV-54 and HPV-70 being the most frequently detected (17; 11% and 10; 6%, respectively). Finally 37 women (23%) tested positive for genotypes of unknown oncogenic risk, the most common in this category being HPV-83 (20; 12%). Multiple infections were detected in 35 (21%) women. By multivariate analysis, HPV genital shedding was significantly associated with young age (OR: 0.34; P
Original languageEnglish
Pages (from-to)430-8
Number of pages9
JournalJournal of Medical Virology
Volume77
Issue number3
DOIs
Publication statusPublished - Nov 2005

Keywords

  • Adolescent
  • Adult
  • Amino Acid Sequence
  • Cervix Uteri
  • DNA, Viral
  • Female
  • Gabon
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Papillomaviridae
  • Papillomavirus Infections
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Prevalence
  • Risk Factors
  • Uterine Cervical Neoplasms
  • Vagina
  • Viral Vaccines
  • Virus Shedding

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