High-resolution analysis of cis-acting regulatory networks at the α-globin locus

Jim R Hughes, Karen M Lower, Ian Dunham, Stephen Taylor, Marco De Gobbi, Jacqueline A Sloane-Stanley, Simon McGowan, Jiannis Ragoussis, Douglas Vernimmen, Richard J Gibbons, Douglas R Higgs

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.
Original languageEnglish
Article number20120361
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume368
Issue number1620
DOIs
Publication statusPublished - 2013

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