High resolution mapping of chromosomal regions controlling resistance to gastrointestinal nematode infections in an advanced intercross line of mice

Jerzy M Behnke, Fuad A Iraqi, John M Mugambi, Simon Clifford, Sonal Nagda, Derek Wakelin, Stephen J Kemp, R Leyden Baker, John P Gibson

Research output: Contribution to journalArticlepeer-review

Abstract

Fine mapping of quantitative trait loci (QTL) associated with resistance to the gastrointestinal parasite Heligmosomoides polygyrus was achieved on F(6)/F(7) offspring (1076 mice) from resistant (SWR) and susceptible (CBA) mouse strains by selective genotyping (top and bottom 20% selected on total worm count in week 6). Fecal egg counts were recorded at weeks 2, 4, and 6, and the average was also analyzed. Blood packed cell volume in weeks 3 and 6 and five immunological traits (mucosal mast cell protease 1, granuloma score, IgG1 against adult worm, IgG1, and IgE to L4 antigen) were also recorded. On Chromosome 1 single-trait analyses identified a QTL with effects on eight traits located at about 24 cM on the F(2) mouse genome database (MGD) linkage map, with a 95% confidence interval (CI) of 20-32 cM established from a multitrait analysis. On Chromosome 17 a QTL with effects on nine traits was located at about 18 cM on the MGD map (CI 17.9-18.4 cM). Strong candidate genes for the QTL position on Chromosome 1 include genes known to be involved in regulating immune responses and on Chromosome 17 genes within the MHC, notably the Class II molecules and tumor necrosis factor.

Original languageEnglish
Pages (from-to)584-97
Number of pages14
JournalMammalian Genome
Volume17
Issue number6
DOIs
Publication statusPublished - Jun 2006

Keywords

  • Animals
  • Crosses, Genetic
  • Gastrointestinal Tract
  • Immunity, Innate
  • Mice
  • Mice, Inbred CBA
  • Nematospiroides dubius
  • Physical Chromosome Mapping
  • Quantitative Trait Loci
  • Strongylida Infections

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