High-resolution structures of a chitinase complexed with natural product cyclopentapeptide inhibitors: Mimicry of carbohydrate substrate

Douglas R. Houston, Kazuro Shiomi, Noriko Arai, Satoshi Omura, Martin G. Peter, Andreas Turberg, Bjørnar Synstad, Vincent G.H. Eijsink, Daan M.F. Van Aalten*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Over the past years, family 18 chitinases have been validated as potential targets for the design of drugs against human pathogens that contain or interact with chitin during their normal life cycles. Thus far, only one potent chitinase inhibitor has been described in detail, the pseudotrisaccharide allosamidin. Recently, however, two potent natural-product cyclopentapeptide chitinase inhibiors, argifin and argadin, were reported. Here, we describe high-resolution crystal structures that reveal the details of the interacions of these cyclopeptides with a family 18 chitinase. The structures are examples of complexes of a carbohydrate processing enzyme with high-affinity peptide-based inhibitors and show in detail how the peptide backbone and side chains mimic the interactions of the enzyme with chitooligosaccharides. Together with enzymological characterization, the structures explain why argadin shows an order of magnitude stronger inhibition than allosamidin, whereas argifin shows weaker inhibition. The pepides bind to the chitinase in remarkably different ways, which may explain the differences in inhibition constants. The two complexes provide a basis for structure-based design of potent chitinase inhibitors, accessible by standard peptide chemistry.

Original languageEnglish
Pages (from-to)9127-9132
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume99
Issue number14
DOIs
Publication statusPublished - 9 Jul 2002

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