High speed spectral fluorescence lifetime imaging for life science applications

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an achromatic confocal laser scanning system capable of fully spectral fluorescence lifetime images at world leading rates (>8 frames per second for a 128x128 image). Imaging is diffraction limited and the Field of View can be changed on the fly. The imaging system includes a single photon avalanche diode line array which generates lifetime histograms on-chip with up to 32 lifetime bins and 512 spectral bins. This sensor can process over 16.5 Giga events/s enabling the system to reach extremely fast spectral lifetime data acquisition, in contrast to the traditional time-correlated single photon counting method.
Original languageEnglish
Title of host publicationPhotonicsWest Paper 10889-25
Subtitle of host publicationHigh-Speed Biomedical Imaging and Spectroscopy IV
PublisherSPIE
DOIs
Publication statusPublished - 2019

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