High speed spectral fluorescence lifetime imaging for life science applications

Elvira Williams; Gareth Williams; Ahmet Erdogan; Neil Finlayson; Robert Henderson; J. M. Girkin

doi:10.1117/12.2509466

High speed spectral fluorescence lifetime imaging for life science applications

Elvira Williams, Gareth Williams, Ahmet Erdogan, Neil Finlayson, Robert Henderson, J. M. Girkin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present an achromatic confocal laser scanning system capable of fully spectral fluorescence lifetime images at world leading rates (>8 frames per second for a 128x128 image). Imaging is diffraction limited and the Field of View can be changed on the fly. The imaging system includes a single photon avalanche diode line array which generates lifetime histograms on-chip with up to 32 lifetime bins and 512 spectral bins. This sensor can process over 16.5 Giga events/s enabling the system to reach extremely fast spectral lifetime data acquisition, in contrast to the traditional time-correlated single photon counting method.

Original language	English
Title of host publication	PhotonicsWest Paper 10889-25
Subtitle of host publication	High-Speed Biomedical Imaging and Spectroscopy IV
Publisher	SPIE
DOIs	https://doi.org/10.1117/12.2509466
Publication status	Published - 2019

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10.1117/12.2509466

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title = "High speed spectral fluorescence lifetime imaging for life science applications",

abstract = "We present an achromatic confocal laser scanning system capable of fully spectral fluorescence lifetime images at world leading rates (>8 frames per second for a 128x128 image). Imaging is diffraction limited and the Field of View can be changed on the fly. The imaging system includes a single photon avalanche diode line array which generates lifetime histograms on-chip with up to 32 lifetime bins and 512 spectral bins. This sensor can process over 16.5 Giga events/s enabling the system to reach extremely fast spectral lifetime data acquisition, in contrast to the traditional time-correlated single photon counting method.",

author = "Elvira Williams and Gareth Williams and Ahmet Erdogan and Neil Finlayson and Robert Henderson and Girkin, {J. M.}",

note = "Acceptance date set to exclude from REF OA Policy",

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AU - Williams, Gareth

AU - Erdogan, Ahmet

AU - Finlayson, Neil

AU - Henderson, Robert

AU - Girkin, J. M.

N1 - Acceptance date set to exclude from REF OA Policy

PY - 2019

Y1 - 2019

N2 - We present an achromatic confocal laser scanning system capable of fully spectral fluorescence lifetime images at world leading rates (>8 frames per second for a 128x128 image). Imaging is diffraction limited and the Field of View can be changed on the fly. The imaging system includes a single photon avalanche diode line array which generates lifetime histograms on-chip with up to 32 lifetime bins and 512 spectral bins. This sensor can process over 16.5 Giga events/s enabling the system to reach extremely fast spectral lifetime data acquisition, in contrast to the traditional time-correlated single photon counting method.

AB - We present an achromatic confocal laser scanning system capable of fully spectral fluorescence lifetime images at world leading rates (>8 frames per second for a 128x128 image). Imaging is diffraction limited and the Field of View can be changed on the fly. The imaging system includes a single photon avalanche diode line array which generates lifetime histograms on-chip with up to 32 lifetime bins and 512 spectral bins. This sensor can process over 16.5 Giga events/s enabling the system to reach extremely fast spectral lifetime data acquisition, in contrast to the traditional time-correlated single photon counting method.

U2 - 10.1117/12.2509466

DO - 10.1117/12.2509466

M3 - Conference contribution

BT - PhotonicsWest Paper 10889-25

PB - SPIE

ER -