Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

Saw Lin Oo, Shishir Venkatesh, Vaithinathan Karthikeyan, Clement Manohar Arava, Spoorthy Pathikonda, Peter K N Yu, Terrence C K Lau, Michael Chen, Vellaisamy A.L. Roy

Research output: Contribution to journalArticlepeer-review

Abstract

Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 uAU-1 mL cm-2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.
Original languageEnglish
Article number2639
JournalSensors
Volume21
Issue number8
DOIs
Publication statusPublished - 9 Apr 2021

Keywords

  • polycarbonate membrane
  • cancer antigen
  • Oligo RNA
  • cancer early detection
  • electrical current sensor

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