Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii

A. Molnar; Andrew Bassett; Eva Thuenemann; Frank Schwach; Shantanu Karkare; Stephan Ossowski; Detlef Weigel; David Baulcombe

doi:10.1111/j.1365-313X.2008.03767.x

Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii

A. Molnar, Andrew Bassett, Eva Thuenemann, Frank Schwach, Shantanu Karkare, Stephan Ossowski, Detlef Weigel, David Baulcombe

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.

Original language	English
Pages (from-to)	165-174
Number of pages	10
Journal	The Plant Journal
Volume	58
Issue number	1
Early online date	19 Jan 2009
DOIs	https://doi.org/10.1111/j.1365-313X.2008.03767.x
Publication status	Published - Apr 2009

Keywords / Materials (for Non-textual outputs)

RNA silencing
artificial miRNA
oligonucleotide cloning
Chlamydomonas
functional genomics

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10.1111/j.1365-313X.2008.03767.xLicence: All Rights Reserved

Cite this

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title = "Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii",

abstract = "MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.",

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AU - Molnar, A.

AU - Bassett, Andrew

AU - Thuenemann, Eva

AU - Schwach, Frank

AU - Karkare, Shantanu

AU - Ossowski, Stephan

AU - Weigel, Detlef

AU - Baulcombe, David

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AB - MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site-specific cleavage of target mRNA with miRNA-complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high-throughput gene silencing in green algae.

KW - RNA silencing

KW - artificial miRNA

KW - oligonucleotide cloning

KW - Chlamydomonas

KW - functional genomics

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DO - 10.1111/j.1365-313X.2008.03767.x

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Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii

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Keywords / Materials (for Non-textual outputs)

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