Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

Nicolaos Avlonitis; Manuelle Debunne; Tashfeen Aslam; Neil McDonald; Chris Haslett; Kevin Dhaliwal; Mark Bradley

doi:10.1039/c3ob40212f

Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

Nicolaos Avlonitis, Manuelle Debunne, Tashfeen Aslam, Neil McDonald, Chris Haslett, Kevin Dhaliwal, Mark Bradley^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.

Original language	English
Pages (from-to)	4414-4418
Number of pages	5
Journal	Organic & Biomolecular chemistry
Volume	11
Issue number	26
DOIs	https://doi.org/10.1039/c3ob40212f
Publication status	Published - 14 Jul 2013

Keywords / Materials (for Non-textual outputs)

CATHEPSIN-G
PROTEINASE-3
ENHANCEMENT
DENDRIMERS

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10.1039/c3ob40212f

http://pubs.rsc.org/en/content/articlehtml/2013/ob/c3ob40212f

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title = "Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase",

abstract = "Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.",

keywords = "CATHEPSIN-G, PROTEINASE-3, ENHANCEMENT, DENDRIMERS",

author = "Nicolaos Avlonitis and Manuelle Debunne and Tashfeen Aslam and Neil McDonald and Chris Haslett and Kevin Dhaliwal and Mark Bradley",

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T1 - Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

AU - Avlonitis, Nicolaos

AU - Debunne, Manuelle

AU - Aslam, Tashfeen

AU - McDonald, Neil

AU - Haslett, Chris

AU - Dhaliwal, Kevin

AU - Bradley, Mark

PY - 2013/7/14

Y1 - 2013/7/14

N2 - Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.

AB - Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.

KW - CATHEPSIN-G

KW - PROTEINASE-3

KW - ENHANCEMENT

KW - DENDRIMERS

U2 - 10.1039/c3ob40212f

DO - 10.1039/c3ob40212f

M3 - Article

SN - 1477-0520

VL - 11

SP - 4414

EP - 4418

JO - Organic & Biomolecular chemistry

JF - Organic & Biomolecular chemistry

IS - 26

ER -

Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase

Abstract

Keywords / Materials (for Non-textual outputs)

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