Hippocampal neurogenesis requires cell-autonomous thyroid hormone signaling

Steffen Mayerl, Heike Heuer, Charles ffrench-Constant

Research output: Contribution to journalArticlepeer-review

Abstract

Adult hippocampal neurogenesis is strongly dependent on thyroid hormone (TH). Whether TH signaling regulates this process in a cell-autonomous or non-autonomous manner remains unknown. To answer this question, we used global and conditional knockouts of the TH transporter monocarboxylate transporter 8 (MCT8), having first used FACS and immunohistochemistry to demonstrate that MCT8 is the only TH transporter expressed on neuroblasts and adult slice cultures to confirm a necessary role for MCT8 in neurogenesis. Both mice with a global deletion or an adult neural stem cell-specific deletion of MCT8 showed decreased expression of the cell-cycle inhibitor P27KIP1, reduced differentiation of neuroblasts, and impaired generation of new granule cell neurons, with global knockout mice also showing enhanced neuroblast proliferation. Together, our results reveal a cell-autonomous role for TH signaling in adult hippocampal neurogenesis alongside non-cell-autonomous effects on cell proliferation earlier in the lineage.
Original languageEnglish
Pages (from-to)845-860
Number of pages16
JournalStem Cell Reports
Volume14
Issue number5
Early online date16 Apr 2020
DOIs
Publication statusPublished - 12 May 2020

Fingerprint Dive into the research topics of 'Hippocampal neurogenesis requires cell-autonomous thyroid hormone signaling'. Together they form a unique fingerprint.

Cite this