HIV-1-infected CD8+CD4+ T cells decay in vivo at a similar rate to infected CD4 T cells during HAART

Gareth J. Hughes, Alexandra Cochrane, Clifford Leenc, Sheila Morris, Jeanne E. Bell, Peter Simmonds

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To investigate the potential for CD4+CD8+ T cells [CD8 double positive (CD8 DP)] T cells to form a reservoir of HIV-1 following HAART through measurement of the rate of decay of infected CD4/CD8 DP T cells.

Methods: HIV-1 proviral loads in highly pure CD4 and CD8 DP T cells were determined for study subjects before and after 200-400 days of therapy and HIV-1 DNA decay rates were calculated.

Results: Before therapy, HIV-1 proviral load in CD8 DP correlated negatively with CD4 cell count. Decay rates of HIV-1-infected CD4 and CD8 DP T cells were similar. Rates for CD8 DP T cells correlated with the time to suppression of viral replication, whereas no such relationship was true for CD4 cell decay rates. A significant reduction in activated cells was observed for both cell types. The action of HAART on HIV-1 replication was similar for both CD4 cells and CD8 DP T cells, although the rate of clearance of infected CD8 DP T cells appeared more critical for a rapid reduction in plasma viral load. Although the size of the CD8 DP T cell reservoir in peripheral blood was smaller relative to that of CD4 cells, HAART did not completely clear HIV-1 infection from this cell subset.

Conclusion: This study confirmed that CD8 DP T cells area major reservoir for HIV-1 in vivo and, therefore, represent a potential reservoir for HIV-1 during HAART, in a manner analogous to that of CD4 T cells. (c) 2008 Wolters Kluwer Health.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalAIDS
Volume22
Issue number1
DOIs
Publication statusPublished - 2 Jan 2008

Keywords

  • Viral Load
  • Lymphocyte Count
  • Anti-Retroviral Agents
  • HIV Infections
  • Humans
  • Antiretroviral Therapy, Highly Active
  • CD4-CD8 Ratio
  • HIV-1
  • CD4-Positive T-Lymphocytes
  • T-Lymphocyte Subsets

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