Abstract / Description of output
The protein transduction domain (PTD) of the HIV-1 Tat protein can facilitate the cellular and nuclear uptake of macromolecular particles. Here, we demonstrate that incorporation without covalent linkage of a 17-amino acid PTD peptide into gene delivery lipoplexes improves gene transfer. Tat/Liposome/DNA (TLD) transfection, as evaluated by Fluorescence Activated Cell Scan analysis of a Green Fluorescence Protein expression plasmid, enabled transfection of highly recalcitrant primary cells in the form of air/liquid interface cultures of sheep tracheal epithelium. Treatment with chloroquine increased, and incubation at low temperature decreased, TLD transfection, suggesting that the endocytosis uptake pathway is involved.
Original language | English |
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Pages (from-to) | 435-444 |
Number of pages | 10 |
Journal | Journal of Controlled Release |
Volume | 99 |
Issue number | 3 |
Publication status | Published - 2004 |
Keywords / Materials (for Non-textual outputs)
- Amino Acid Sequence Animals Carrier Proteins/genetics/pharmacokinetics Cell Line, Tumor Chloroquine/pharmacology DNA/genetics/metabolism DNA-Binding Proteins/genetics/metabolism Deoxyribonuclease I/drug effects/physiology Drug Evaluation, Preclinical/methods Electrophoretic Mobility Shift Assay/methods Endocytosis/drug effects/physiology Fatty Acids, Monounsaturated/chemistry/pharmacokinetics Flow Cytometry/methods Forecasting Gene Products, tat/genetics/*pharmacokinetics Gene Therapy/methods Genetic Vectors/genetics/pharmacokinetics HIV-1/genetics/*physiology Humans Liposomes/chemistry/*pharmacokinetics Macromolecular Substances/chemistry Particle Size Phosphatidylethanolamines/pharmacokinetics Plasmids/chemistry/pharmacokinetics Quaternary Ammonium Compounds/chemistry/pharmacokinetics Sequence Analysis, Protein/methods Sheep Trachea/drug effects/pathology/physiology Transfection/*methods tat Gene Products, Human Immunodeficiency Virus