Homeostatic vs inflammatory replenishment of tissue mononuclear phagocytes revealed by fate mapping CSF1R myeloid precursors

Barry Bradford; David Hume; Neil Mabbott

Homeostatic vs inflammatory replenishment of tissue mononuclear phagocytes revealed by fate mapping CSF1R myeloid precursors

Barry Bradford, David Hume, Neil Mabbott

Research output: Contribution to conference › Poster

Abstract

The myelopoeitic precursors in bone marrow give rise to an array of immune cells including monocytes, macrophages, dendritic cells and neutrophils/granulocytes. These cells are distributed throughout the body and perform a variety of functions including homeostatic regulation of tissue and responses to tissue damage or invading pathogens. The precursors for each of these cell types have been increasingly characterized and identified, however many precursor populations reveal pluripotent potential. We performed adoptive precursor transfer of cells sorted on their relative expression of the colony stimulating factor 1 receptor (Csf1r) using the Csf1r-eGFP transgenic mouse model and Gr-1 antibody reactivity and fate-mapped the differentiation of various precursor populations. We observed variability in retention and differentiation of these populations within the bone marrow following transfer into sub-lethally irradiated recipients. The differentiation potentials of the precursor cell populations were modulated by the relative marker expression at the time of transfer.

Csf1r+/Gr-1+ cells failed to populate the bone marrow during inflammatory conditions. Csf1r+/Gr-1- cell recipients revealed eGFP expression in tissue-resident cells marked with homeostatic and inflammatory markers in equal measure. This modulatory effect however was most apparent following transfer of the least differentiated Csf1r-/Gr-1- precursor. In which recipients revealed only homeostatic replenishment of eGFP+ tissue macrophages and dendritic cells despite competition from host-derived (non eGFP) ly-6C+ “inflammatory” monocytes. These data reveal that homeostatic and inflammatory population of tissues by MPS cells can occur via the same precursor population but by independent differentiation pathways. Competitive re-population of tissues occurred between donor derived mononuclear phagocytes and host derived “inflammatory” monocyte and tissue-resident populations. We observed extensive multi-potent potential in pre-Csf1r (pre eGFP) expressing myeloid precursors using eGFP expression to trace their progeny through to tissue-resident mononuclear phagocytes.

Original language	English
Publication status	Published - 17 Jan 2013
Event	EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity - Marseilles, France Duration: 17 Jan 2013 → 19 Jan 2013

Conference

Conference	EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity
Country/Territory	France
City	Marseilles
Period	17/01/13 → 19/01/13

Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research
Csf1 and the control of postnatal growth and organ development in the rat
Hume, D. (Principal Investigator), Burdon, T. (Co-investigator), Farquharson, C. (Co-investigator) & Whitelaw, B. (Co-investigator)
MRC
12/07/10 → 11/06/14
Project: Research
Determining the role of cxcr5-expressing dendritic cells in imune function and tse agent neuroinvasion from the intestine
Mabbott, N. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/05/09 → 30/09/12
Project: Research

EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity
Bradford, B. (Participant)
17 Jan 2013 → 19 Jan 2013
Activity: Participating in or organising an event types › Participation in workshop, seminar, course

Cite this

@conference{0df30502ac9949c99cab36a975c02842,

title = "Homeostatic vs inflammatory replenishment of tissue mononuclear phagocytes revealed by fate mapping CSF1R myeloid precursors",

abstract = "The myelopoeitic precursors in bone marrow give rise to an array of immune cells including monocytes, macrophages, dendritic cells and neutrophils/granulocytes. These cells are distributed throughout the body and perform a variety of functions including homeostatic regulation of tissue and responses to tissue damage or invading pathogens. The precursors for each of these cell types have been increasingly characterized and identified, however many precursor populations reveal pluripotent potential. We performed adoptive precursor transfer of cells sorted on their relative expression of the colony stimulating factor 1 receptor (Csf1r) using the Csf1r-eGFP transgenic mouse model and Gr-1 antibody reactivity and fate-mapped the differentiation of various precursor populations. We observed variability in retention and differentiation of these populations within the bone marrow following transfer into sub-lethally irradiated recipients. The differentiation potentials of the precursor cell populations were modulated by the relative marker expression at the time of transfer. Csf1r+/Gr-1+ cells failed to populate the bone marrow during inflammatory conditions. Csf1r+/Gr-1- cell recipients revealed eGFP expression in tissue-resident cells marked with homeostatic and inflammatory markers in equal measure. This modulatory effect however was most apparent following transfer of the least differentiated Csf1r-/Gr-1- precursor. In which recipients revealed only homeostatic replenishment of eGFP+ tissue macrophages and dendritic cells despite competition from host-derived (non eGFP) ly-6C+ “inflammatory” monocytes. These data reveal that homeostatic and inflammatory population of tissues by MPS cells can occur via the same precursor population but by independent differentiation pathways. Competitive re-population of tissues occurred between donor derived mononuclear phagocytes and host derived “inflammatory” monocyte and tissue-resident populations. We observed extensive multi-potent potential in pre-Csf1r (pre eGFP) expressing myeloid precursors using eGFP expression to trace their progeny through to tissue-resident mononuclear phagocytes. ",

author = "Barry Bradford and David Hume and Neil Mabbott",

year = "2013",

month = jan,

day = "17",

language = "English",

note = "EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity ; Conference date: 17-01-2013 Through 19-01-2013",

}

TY - CONF

T1 - Homeostatic vs inflammatory replenishment of tissue mononuclear phagocytes revealed by fate mapping CSF1R myeloid precursors

AU - Bradford, Barry

AU - Hume, David

AU - Mabbott, Neil

PY - 2013/1/17

Y1 - 2013/1/17

N2 - The myelopoeitic precursors in bone marrow give rise to an array of immune cells including monocytes, macrophages, dendritic cells and neutrophils/granulocytes. These cells are distributed throughout the body and perform a variety of functions including homeostatic regulation of tissue and responses to tissue damage or invading pathogens. The precursors for each of these cell types have been increasingly characterized and identified, however many precursor populations reveal pluripotent potential. We performed adoptive precursor transfer of cells sorted on their relative expression of the colony stimulating factor 1 receptor (Csf1r) using the Csf1r-eGFP transgenic mouse model and Gr-1 antibody reactivity and fate-mapped the differentiation of various precursor populations. We observed variability in retention and differentiation of these populations within the bone marrow following transfer into sub-lethally irradiated recipients. The differentiation potentials of the precursor cell populations were modulated by the relative marker expression at the time of transfer. Csf1r+/Gr-1+ cells failed to populate the bone marrow during inflammatory conditions. Csf1r+/Gr-1- cell recipients revealed eGFP expression in tissue-resident cells marked with homeostatic and inflammatory markers in equal measure. This modulatory effect however was most apparent following transfer of the least differentiated Csf1r-/Gr-1- precursor. In which recipients revealed only homeostatic replenishment of eGFP+ tissue macrophages and dendritic cells despite competition from host-derived (non eGFP) ly-6C+ “inflammatory” monocytes. These data reveal that homeostatic and inflammatory population of tissues by MPS cells can occur via the same precursor population but by independent differentiation pathways. Competitive re-population of tissues occurred between donor derived mononuclear phagocytes and host derived “inflammatory” monocyte and tissue-resident populations. We observed extensive multi-potent potential in pre-Csf1r (pre eGFP) expressing myeloid precursors using eGFP expression to trace their progeny through to tissue-resident mononuclear phagocytes.

AB - The myelopoeitic precursors in bone marrow give rise to an array of immune cells including monocytes, macrophages, dendritic cells and neutrophils/granulocytes. These cells are distributed throughout the body and perform a variety of functions including homeostatic regulation of tissue and responses to tissue damage or invading pathogens. The precursors for each of these cell types have been increasingly characterized and identified, however many precursor populations reveal pluripotent potential. We performed adoptive precursor transfer of cells sorted on their relative expression of the colony stimulating factor 1 receptor (Csf1r) using the Csf1r-eGFP transgenic mouse model and Gr-1 antibody reactivity and fate-mapped the differentiation of various precursor populations. We observed variability in retention and differentiation of these populations within the bone marrow following transfer into sub-lethally irradiated recipients. The differentiation potentials of the precursor cell populations were modulated by the relative marker expression at the time of transfer. Csf1r+/Gr-1+ cells failed to populate the bone marrow during inflammatory conditions. Csf1r+/Gr-1- cell recipients revealed eGFP expression in tissue-resident cells marked with homeostatic and inflammatory markers in equal measure. This modulatory effect however was most apparent following transfer of the least differentiated Csf1r-/Gr-1- precursor. In which recipients revealed only homeostatic replenishment of eGFP+ tissue macrophages and dendritic cells despite competition from host-derived (non eGFP) ly-6C+ “inflammatory” monocytes. These data reveal that homeostatic and inflammatory population of tissues by MPS cells can occur via the same precursor population but by independent differentiation pathways. Competitive re-population of tissues occurred between donor derived mononuclear phagocytes and host derived “inflammatory” monocyte and tissue-resident populations. We observed extensive multi-potent potential in pre-Csf1r (pre eGFP) expressing myeloid precursors using eGFP expression to trace their progeny through to tissue-resident mononuclear phagocytes.

M3 - Poster

T2 - EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity

Y2 - 17 January 2013 through 19 January 2013

ER -

Homeostatic vs inflammatory replenishment of tissue mononuclear phagocytes revealed by fate mapping CSF1R myeloid precursors

Abstract

Conference

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Projects

Innate immunity and endemic diseases in livestock species

Csf1 and the control of postnatal growth and organ development in the rat

Determining the role of cxcr5-expressing dendritic cells in imune function and tse agent neuroinvasion from the intestine

Activities

EMBO 2013 - Dr Jekyll and Mr Hyde: The Macrophage in Inflammation and Immunity

Cite this