Projects per year
Abstract
The chromosomal passenger complex (CPC) is directed to centromeres during mitosis via binding to H3T3ph and Sgo1. Whether and how heterochromatin protein 1α (HP1α) influences CPC localisation and function during mitotic entry is less clear. Here, we alter HP1α dynamics by fusing it to a CENP-B DNA-binding domain. Tethered HP1 strongly recruits the CPC, destabilising kinetochore-microtubule interactions and activating the spindle assembly checkpoint. During mitotic exit, the tethered HP1 traps active CPC at centromeres. These HP1-CPC clusters remain catalytically active throughout the subsequent cell cycle. We also detect interactions between endogenous HP1 and the CPC during G2HP1α and HP1γ cooperate to recruit the CPC to active foci in a CDK1-independent process. Live cell tracking with Fab fragments reveals that H3S10ph appears well before H3T3 is phosphorylated by Haspin kinase. Our results suggest that HP1 may concentrate and activate the CPC at centromeric heterochromatin in G2before Aurora B-mediated phosphorylation of H3S10 releases HP1 from chromatin and allows pathways dependent on H3T3ph and Sgo1 to redirect the CPC to mitotic centromeres.
Original language | English |
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Number of pages | 18 |
Journal | EMBO Journal |
Volume | 37 |
Issue number | 6 |
Early online date | 21 Feb 2018 |
DOIs | |
Publication status | Published - 15 Mar 2018 |
Keywords / Materials (for Non-textual outputs)
- CENP‐B
- Chromosomal Passenger Complex
- Heterochromatin Protein 1
- Histone H3S10 Phosphorylation
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Dive into the research topics of 'HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry'. Together they form a unique fingerprint.Projects
- 3 Finished
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Molecular Mechanisms of Centromere Inheritance and Kinetochore Function
4/01/17 → 4/10/22
Project: Research
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The role of non-histone proteins in chromosome structure and function during mitosis
1/10/15 → 30/09/21
Project: Research
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R43494 Advancing our capabilities for visualization of cellular structures and isolated complexes byelectron microscopy
Böttcher, B. & Schirmer, E.
22/06/15 → 21/06/20
Project: Research