Abstract
MSP2 is a merozoite surface protein of Plasmodium falciparum and, as such, is a potential component of a malaria vaccine. In this study, we have used a panel of recombinant MSP2 antigens in enzyme-linked immunosorbent assays to investigate the recognition of MSP2 by antibodies from malaria-immune human serum. These recombinant antigens include full-length proteins of serogroups A and B and fragments representing the conserved, group-specific, or repeat regions of each serogroup. Ninety-five percent of the serum samples tested contained MSP2-specific antibodies: 81% of serum samples tested responded to serogroup A, and 86% responded to serogroup B. The antibody response is directed almost exclusively towards dimorphic and polymorphic regions of MSP2; the conserved regions are rarely recognized, and antibodies to serogroups A and B do not cross-react. Interestingly, the antibody response is predominately of the cytophilic and complement-fixing subclass immunoglobulin G3.
Original language | English |
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Pages (from-to) | 4382-8 |
Number of pages | 7 |
Journal | Infection and Immunity |
Volume | 63 |
Issue number | 11 |
Publication status | Published - Nov 1995 |
Keywords / Materials (for Non-textual outputs)
- Amino Acid Sequence
- Animals
- Antibodies, Protozoan
- Antigens, Protozoan
- Cross Reactions
- Humans
- Immunoglobulin G
- Malaria, Falciparum
- Molecular Sequence Data
- Plasmodium falciparum
- Protozoan Proteins
- Recombinant Fusion Proteins
- Serotyping