Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology

Natalay Kouprina; Alexey N. Tomilin; Hiroshi Masumoto; William C. Earnshaw; Vladimir Larionov

doi:10.1517/17425247.2014.882314

Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology

Natalay Kouprina^*, Alexey N. Tomilin, Hiroshi Masumoto, William C. Earnshaw, Vladimir Larionov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: Human artificial chromosomes (HACs) have several advantages over viruses as gene delivery vectors, including stable episomal maintenance in a single copy and the ability to carry large gene inserts. Areas covered: In this review, we summarise recent work on gene transfer into mammalian cells using the HACs. HACs allow therapeutic transgenes to be expressed in target cells under conditions that recapitulate the physiological regulation of endogenous loci. Expert opinion: Based on the published data, the HAC vectors have a great potential for gene therapy, regenerative medicine, screening of anticancer drugs and biotechnology.

Original language	English
Pages (from-to)	517-535
Number of pages	19
Journal	Expert Opinion on Drug Delivery
Volume	11
Issue number	4
DOIs	https://doi.org/10.1517/17425247.2014.882314
Publication status	Published - 1 Apr 2014

Keywords / Materials (for Non-textual outputs)

Gene delivery vector
Gene expression
Gene therapy
Human artificial chromosomes
Insulators
TAR cloning

Access to Document

10.1517/17425247.2014.882314

Cite this

@article{3225ac5567aa49109b6eec8c337b4eea,

title = "Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology",

abstract = "Introduction: Human artificial chromosomes (HACs) have several advantages over viruses as gene delivery vectors, including stable episomal maintenance in a single copy and the ability to carry large gene inserts. Areas covered: In this review, we summarise recent work on gene transfer into mammalian cells using the HACs. HACs allow therapeutic transgenes to be expressed in target cells under conditions that recapitulate the physiological regulation of endogenous loci. Expert opinion: Based on the published data, the HAC vectors have a great potential for gene therapy, regenerative medicine, screening of anticancer drugs and biotechnology.",

keywords = "Gene delivery vector, Gene expression, Gene therapy, Human artificial chromosomes, Insulators, TAR cloning",

author = "Natalay Kouprina and Tomilin, {Alexey N.} and Hiroshi Masumoto and Earnshaw, {William C.} and Vladimir Larionov",

year = "2014",

month = apr,

day = "1",

doi = "10.1517/17425247.2014.882314",

language = "English",

volume = "11",

pages = "517--535",

journal = "Expert Opinion on Drug Delivery",

issn = "1742-5247",

publisher = "Informa Healthcare",

number = "4",

}

TY - JOUR

T1 - Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology

AU - Kouprina, Natalay

AU - Tomilin, Alexey N.

AU - Masumoto, Hiroshi

AU - Earnshaw, William C.

AU - Larionov, Vladimir

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Introduction: Human artificial chromosomes (HACs) have several advantages over viruses as gene delivery vectors, including stable episomal maintenance in a single copy and the ability to carry large gene inserts. Areas covered: In this review, we summarise recent work on gene transfer into mammalian cells using the HACs. HACs allow therapeutic transgenes to be expressed in target cells under conditions that recapitulate the physiological regulation of endogenous loci. Expert opinion: Based on the published data, the HAC vectors have a great potential for gene therapy, regenerative medicine, screening of anticancer drugs and biotechnology.

AB - Introduction: Human artificial chromosomes (HACs) have several advantages over viruses as gene delivery vectors, including stable episomal maintenance in a single copy and the ability to carry large gene inserts. Areas covered: In this review, we summarise recent work on gene transfer into mammalian cells using the HACs. HACs allow therapeutic transgenes to be expressed in target cells under conditions that recapitulate the physiological regulation of endogenous loci. Expert opinion: Based on the published data, the HAC vectors have a great potential for gene therapy, regenerative medicine, screening of anticancer drugs and biotechnology.

KW - Gene delivery vector

KW - Gene expression

KW - Gene therapy

KW - Human artificial chromosomes

KW - Insulators

KW - TAR cloning

U2 - 10.1517/17425247.2014.882314

DO - 10.1517/17425247.2014.882314

M3 - Article

C2 - 24479793

AN - SCOPUS:84897857602

SN - 1742-5247

VL - 11

SP - 517

EP - 535

JO - Expert Opinion on Drug Delivery

JF - Expert Opinion on Drug Delivery

IS - 4

ER -

Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Cite this