Abstract
We have developed a method to bulk culture definitive endoderm cells generated from human iPSCs which can be stored and differentiated to hepatocytes. Human iPSC-derived definitive endoderm cells were sorted based on the expression of CXCR4. The sorted cells were able to proliferate for extended periods and can be cryopreserved. The definitive endoderm cells were subsequently utilized to generate functional hepatocytes expressing albumin and α-fetoprotein in different multiwell formats. This provides a method to reliably produce more consistent hepatocytes in greater quantities and has enabled the development of high-throughput screening strategies.
Original language | English |
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Pages (from-to) | 41-53 |
Number of pages | 13 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1994 |
DOIs | |
Publication status | Published - 24 May 2019 |
Keywords / Materials (for Non-textual outputs)
- Cell Culture Techniques/methods
- Cell Differentiation/drug effects
- Cell Separation/methods
- Cells, Cultured
- Endoderm/cytology
- Fetal Proteins/metabolism
- Flow Cytometry
- Hepatocytes/cytology
- Humans
- Induced Pluripotent Stem Cells/cytology
- Receptors, CXCR4/metabolism
- Serum Albumin, Human/metabolism